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1D). We verified that this is due to a defect in translocation rather than reduced biosynthesis by using a construct in which glutathione S-transferase (GST) had been fused to the N-terminus of the Gls1 SP, thus increasing the size of the fragment cleaved off by signal peptidase upon ...
(c) Total cellular GST activities were determined using the same cell lysates in (b) (n ¼ 3–4, ±S.E.M.). Significant differences between untreated Txnrd1fl/fl without additional selenite and the other samples are indicated (*Po0.05; n.s., not significant, P40.05) protective ...
SIL9489ACTUC、VI-J7L-IZ、TLVH431ILPR、L4949DG、AT25020N-SC、A7721、JK0-0145NL、LLQ1608-F39NG、SFI1812ML470C、SI-7330A、HRPDAF025、NCS6433DTBR2G、M27C1001-20C1、LH1208AAJ、DMG7408SFG-7、STB80NE06-10、C3072、LV5051T-TLM-E、MC68230CFN10、GP4504MTR-G1、RF7244KSR、CR75NP-1R5MC...
c Interaction between purified CIDEC/FSP27 and GST-Flag-tagged SNAREs using an in vitro GST pull-down assay followed by western blot. d Lipid mixing between adiposomes was measured from the development of FRET between NBD-labeled lipids and Rhod-labeled lipids. The NBD-adiposomes were ...
Sec22-GST fusion protein, or GST alone, was incubated with wild-type Sec23/24a or mutant Sec23/24a-R541A (at concentrations from 1.25 to 20 μM), as described in Experimental Procedures. Bound proteins were analyzed by 4%–15% SDS-PAGE and Coomassie blue staining. (D) Binding of ...
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