Sso2 Input + + –––– His6-Pep12 (50%) –– + + –– GST-Sec3N GST-Exo70C His6-Sso2 His6-Pep12 Coomassie blue staining d 100 75 50 25 0 20 Load Sso2- Sec3N- Sso2 Sec3N Sso2/Sec3N 40 60 80 Volume (ml) Elution fractions 100 Figure 1 | Sec3 interacts with Sso2. ...
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While sbh1S3A/T5A cells accumulated significant amounts of GSTpGls1 in the cytosol (Fig. S4D), none of the proline-directed kinase mutants did (Fig. S4E). Our results suggest that despite their defect in Mns1ΔgpαF translocation, Δkns1,Δmck1, and cdc28-1 cells were competent ...
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(c) Total cellular GST activities were determined using the same cell lysates in (b) (n ¼ 3–4, ±S.E.M.). Significant differences between untreated Txnrd1fl/fl without additional selenite and the other samples are indicated (*Po0.05; n.s., not significant, P40.05) protective ...
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氨基酸大小27kDa 表达氨基酸1-248aa BackgroundMay be involved in thymocyte signaling. ApplicationImmunology research Derived fromE.coli PurificationNI-NTA affinity purification FormulationLyophilized from a 0.2 μm filtered solution of Tris-HCl, PH8.0. ...
Strikingly, the Sec23a/Sec24a-R541A mutant bound to Sec22-GST no more tightly than to GST alone (compare, for example, lanes 15 and 16). These results correlate nicely with those from the packaging assay (Figures 1A and 1B) and establish that the inability of the C site mutant Sec24...