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While sbh1S3A/T5A cells accumulated significant amounts of GSTpGls1 in the cytosol (Fig. S4D), none of the proline-directed kinase mutants did (Fig. S4E). Our results suggest that despite their defect in Mns1ΔgpαF translocation, Δkns1,Δmck1, and cdc28-1 cells were competent ...
SIL9489ACTUC、VI-J7L-IZ、TLVH431ILPR、L4949DG、AT25020N-SC、A7721、JK0-0145NL、LLQ1608-F39NG、SFI1812ML470C、SI-7330A、HRPDAF025、NCS6433DTBR2G、M27C1001-20C1、LH1208AAJ、DMG7408SFG-7、STB80NE06-10、C3072、LV5051T-TLM-E、MC68230CFN10、GP4504MTR-G1、RF7244KSR、CR75NP-1R5MC...
(c) Total cellular GST activities were determined using the same cell lysates in (b) (n ¼ 3–4, ±S.E.M.). Significant differences between untreated Txnrd1fl/fl without additional selenite and the other samples are indicated (*Po0.05; n.s., not significant, P40.05) protective ...
c Interaction between purified CIDEC/FSP27 and GST-Flag-tagged SNAREs using an in vitro GST pull-down assay followed by western blot. d Lipid mixing between adiposomes was measured from the development of FRET between NBD-labeled lipids and Rhod-labeled lipids. The NBD-adiposomes were ...
pCMV-SPORT6-SEC14L4质粒结构是一个大肠杆菌表达载体,Tac强启动子可以驱动GST促溶标签和目的基因融合表达,LacI阻碍蛋白和Laco操作子使本质粒在没有加入IPTG之前禁止表达,防止其影响细菌生长。表达后的融合蛋白可用凝血酶蛋白酶切割,再过一次GST柱子即可清除掉GST标签。 产品相册pCMV-SPORT6-SEC14L4质粒结构TTTCCTGCGTT...
so as to restore its financial health. The PCA Framework is also intended to act as a tool for effective market discipline. The PCA Framework does not preclude the Reserve Bank of India from taking any other action as it deems fit at any time in addition...
(e.g.,WD40, WRKY, LEAandGST), signal transduction-related genes (e.g., receptor kinase) and energy metabolism-related genes (e.g.,Plasma membrane ATPaseandBeta-glucosidase) (Table S1). These proteins have previously been reported to act either directly or indirectly in abiotic stress ...