scNMT-seq enables joint profiling of chromatin accessibility DNA methylation and transcription in single cells. Nat Commun (2018) 9(1):781. doi:10.1038/s41467-018-03149-4Clark SJ, Argelaguet R, Kapourani CA, Stubbs TM, Lee HJ, Alda-Catalinas C, Krueger F, Sanguinetti G, Kelsey G, ...
scNMT-seq Source code of the manuscriptJoint profiling of chromatin accessibility, DNA methylation and transcription in single cells. Abstract Parallel single-cell sequencing protocols represent powerful methods for investigating regulatory relationships, including epigenome-transcriptome interactions. Here, we ...
scNMT-seq robustly profiles each molecular layer To validate scNMT-seq, we applied the method to a batch of 70 serum-grown EL16 mouse embryonic stem cells (ESCs), together with four negative (empty wells) and three scM&T-seq controls (cells processed using scM&T-seq, i.e., without M....
北京大学第三医院乔杰、闫丽盈团队开发了一种单细胞多组学测序技术(scNOMeRe-seq),该技术可同时对单个细胞的RNA表达、DNA甲基化、染色质可及性进行分析。 研究团队利用这一技术首次对哺乳动物植入前胚胎在单细胞分辨率上多个层面的调控关系...