scNMT-seq enables joint profiling of chromatin accessibility DNA methylation and transcription in single cells. Nat Commun (2018) 9(1):781. doi:10.1038/s41467-018-03149-4Clark SJ, Argelaguet R, Kapourani CA, Stubbs TM, Lee HJ, Alda-Catalinas C, Krueger F, Sanguinetti G, Kelsey G, ...
scNMT-seq (single-cell nucleosome, methylation and transcription sequencing) uses a GpC methyltransferase to label open chromatin followed by bisulfite and RNA sequencing. We validate scNMT-seq by applying it to mouse embryonic stem cells and embryoid body cells, finding links between all three ...
scNMT-Seq, CITE-Seq, G&T-Seq, SHARE-Seq, ASAP-Seq, DOGMA-Seq, and also Kitchen-Seq :)) A nice read on the multi-omic explosion in single-cell biology! 发布于 2021-07-24 15:03 4 人喜欢 分享 收藏举报 登录知乎,您可以享受以下权益: ...
北京大学第三医院乔杰、闫丽盈团队开发了一种单细胞多组学测序技术(scNOMeRe-seq),该技术可同时对单个细胞的RNA表达、DNA甲基化、染色质可及性进行分析。 研究团队利用这一技术首次对哺乳动物植入前胚胎在单细胞分辨率上多个层面的调控关系...