Since this tailing reaction can extend to up to 50 bp (Supplementary Fig. 1b), the long synthetic tail sequences were not sufficiently trimmed by snmC- seq trimming parameters (16 bp from both 5′ and 3′) causing a failure in mapping of reverse reads that retained these synthetic tail ...
Here we report snmC-seq2, which provides improved read mapping, reduced artifactual reads, enhanced throughput, as well as increased library complexity and coverage uniformity compared to snmC-seq. snmC-seq2 is an efficient strategy suited for large-scale single-cell epigenomic studies....
snmcseq Tools for computational analysis of single nuclei methylC-Seq data, as described in Luo, Keown et al. (2017) Contact: Chris Keown (ckeown @ ucsd.edu), Eran Mukamel (emukamel @ ucsd.edu) Mukamel lab: http://brainome.ucsd.edu See also: Methylpy https://github.com/yupenghe/meth...