Here, we show that SHARP1 is an unstable protein that is targeted for proteasomal degradation by the E3 ubiquitin ligase complex SCF beta TrCP. SHARP1 recruits beta TrCP via a phosphodegron encompassing Ser240 and Glu245 which are required for SHARP1 ubiquitylation and degradation. Furthermore, ...
The SCFbeta-TRCP complex directly recognizes a characteristic phosphodegron motif in its substrates that depends on antecedent kinase activity. We characterized the beta-TRCP phosphodegrons in both SPAR and REST, and further explored characteristics of other beta-TRCP phosphodegrons found in substrates ...
2003. Prophase destruction of Emi1 by the SCF βTrCP/Slimb ubiquitin ligase activates the anaphase promoting complex to allow progression beyond prometaphase. Dev. Cell 4 : 813-826.Margottin-Goguet F., Hsu J.Y., Loktev A., Hsieh H.M., Reimann J.D.R., Jackson P.K. Prophase ...
Identification of Small Non-Peptidic Ligands that Bind the Scf-beta-TRCP Ubiquitin Ligase to Target to ER for Ubiquitination and DegradationKathleen Sakamoto
Thus, betaTrCP-DEPTOR-mTOR intertwine to regulate cell survival and autophagy.Zhao YXiong XSun YMolecular cellZhao Y, Xiong X, Sun Y (2011) DEPTOR, an mTOR inhibitor, is a physiological substrate of SCF(betaTrCP) E3 ubiquitin ligase and regulates survival and autophagy. Mol Cell 44(2):...
Beta transducin repeat-containing proteinsThere is increasing evidence that histidine triad nucleotide-binding protein 1 (HINT1) is a novel tumor suppressor. In the present study, we investigated the mechanism by which HINT1 promotes the stability of inhibitor of NF-kappa B alpha (I kappa B ...
KunmingZhitianKunmingWangKunmingLinKunmingYangKunmingDigestive Diseases and SciencesHint1 up-regulates Ikappa Balpha by targeting the beta-Tr CP subunit of SCF E3 ligase in human hepatocellular carcinoma cells. Shi Z,Wu X,Ke Y,et al. Digestive Diseases and Sciences . 2016...