细胞在体内紧密的空间排列使得单个细胞之间以及细胞与细胞外基质之间能够进行复杂的相互作用。这种排列为细胞提供了独特的线索,以指导特定的细胞功能和生长。为了在体外复制这种空间排列,可以在三维(3D)培养系统中培养细胞,使细胞与细胞聚集形成球体。让细胞...
Cell-based scaffold-free therapies seek to develop in vitro organotypic three-dimensional (3D) tissue-like surrogates, capitalising upon the inherent capacity of cells to create tissues with efficiency and sophistication that is still unparalleled by human-made devices. Although automation systems have ...
细胞在体内紧密的空间排列使得单个细胞之间以及细胞与细胞外基质之间能够进行复杂的相互作用。这种排列为细胞提供了的线索,以指导特定的细胞功能和生长。为了在体外复制这种空间排列,可以在三维(3D)培养系统中培养细胞,使细胞与细胞聚集形成球体。让细胞聚集成细胞球体的无基质(SCAFFOLD-FREE)3D细胞球体培养是构建3D细胞球...
The cues include a biological growing template, spatial coculture, biomimetic radial flow, and circulation in a scaffold-free condition. The feasibility of recapitulating a multiscale physiological structural hierarchy, complex drug clearance, and zonal physiology from the cell to tissue level in long...
and biological cues are combined. The development of a construct without an artificial scaffold is one of the main concepts of TE technology. A novel tissue-engineering technique has been recently developed for cartilage repair using a scaffold-free tissue-engineered construct (TEC) biosynthesized fro...
Cell-based scaffold-free therapies seek to develop in vitro organotypic three-dimensional (3D) tissue-like surrogates, capitalising upon the inherent capacity of cells to create tissues with efficiency and sophistication that is still unparalleled by human-made devices. Although automation systems have ...
To address these problems, we have developed a scaffold-free method to generate 3D adipose spheroids from primary or immortal human or mouse pre-adipocytes. Pre-adipocytes self-organize into spheroids in hanging drops and upon transfer to low attachment plates, can be maintained in long-term ...
The spheroids were arranged into patches using a Kenzan bio-3D printer to create scaffold-free, cell-only cartilage constructs. Basic fibroblast growth factor (bFGF) was added during expansion culture and varying concentrations of bone morphogenetic protein2 (BMP2) were supplemented during chondrogenic...
Here we show that scaffold-free systems are well suited for the analysis of ECM protein regulation. Using quantitative mass spectrometry-based proteomics in combination with magnetic 3D bioprinting we characterize changes in the proteome of skin fibroblasts and squamous cell carcinoma cells. Transferring...
1.Is scaffold free of makeshift devices or ladders to increase height?脚手架没有为了增加高度安装临时装置或梯子吗？2.The scaffold is meant to suggest the nomadic state of the free store and its ability to be set up anywhere.脚手架是为了显示免费商店的流动状态和能够设于任何地方的特性...