This chapter discusses threeヾimensional (3D) scaffold‐free bioprinting, fourヾimensional (4D) bioprinting, and the recent efforts at 4D scaffold‐free bioprinting. The basic unit in 3D scaffold‐free bioprinting is a multicellular spheroid. When the spheroids are ready, the 3D bioprinter is...
Hybrid bioprinting of Zonally Stratified Human articular cartilage using Scaffold-Free tissue strands as Building blocks. Adv Healthc Mater. 2020;9(22). Fujimoto R, Murata D, Nakayama K. Bio-3D printing of scaffold-free osteogenic and chondrogenic constructs using rat adipose-derived stromal cells....
Ozbolat IT (2015) Scaffold-based or scaffold-free bioprinting: competing or complementing approaches? J Nanotechnol Eng Med 6(2):024701 Article CAS Google Scholar Phillips TJ (1998) New skin for old: developments in biological skin substitutes. Arch Dermatol 134(3):344–349 Article CAS PubMed...
Therefore, a scaffold-free bioprinting approach is gaining more interest [81]. In 2004, Dr. Gabor Forgacs introduced a new scaffold-free technique where hydrogel-encapsulated cells were used to construct tissue blocks of specific geometric patterns to mimic the target tissue or organ [81]. This ...
Billiet and co-workers (2012) define organ bioprinting as ‘the engineering of 3D living structures supported by the self-assembly/organizing capabilities of cells delivered through the application of SFF techniques based on laser, inkjet, or extrusion freeforming technologies’. Inkjet and extrusion-...
Cell-based scaffold-free therapies seek to develop in vitro organotypic three-dimensional (3D) tissue-like surrogates, capitalising upon the inherent capacity of cells to create tissues with efficiency and sophistication that is still unparalleled by human-made devices. Although automation systems have ...
In contrast, an alternative method to generate scaffold-free cartilage by bioprinting and fusing microspheroids of hESC-derived MSCs resulted in a heterogeneous tissue construct displaying a non-uniform population of cells31. Human articular cartilage regularly experiences forces in the region of 18 ...
Wound Healing 3D Bioprinting Immunomodulation Polymeric Scaffolds Micropatterned Surface Microplates Nanofiber Based Scaffolds Scaffold TechnologyDisease Type Outlook (Revenue, USD Million, 2018 - 2030) Orthopedics, Musculoskeletal, & Spine Cancer Skin & Integumentary ...
000 cells per well. The plates were then incubated at 37 ℃ in a 5% CO2 incubator for 48 h. The cells were washed 2–3 times with PBS buffer followed by 2–3 washes with 1 mL of sugar-free Krebs-Ringer Bicarbonate HEPES (KRBH, JingKeHuaXue) buffer containing 0.1% Bovine Serum ...
Y. Design and Printing Strategies in 3D Bioprinting of Cell-Hydrogels: A Review. Adv Healthc Mater. doi: 10.1002/adhm.201600435 (2016). Norotte, C., Marga, F. S., Niklason, L. E. & Forgacs, G. Scaffold-free vascular tissue engineering using bioprinting. Biomaterials 30, 5910–7 (...