To establish relevantin vitromodels mimicking the complex design observedin vivo,scaffold-based and scaffold-free 3D cell culture systems have been developed. Here we show that scaffold-free systems are well suited for the analysis of ECM protein regulation. Using quantitative mass spectrometry-based ...
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59]. Different approaches for 3D cell cultures exist and can be generally divided into scaffold-based and scaffold-free methods. Scaffold-free 3D cell culture refers to a cell culture technique in which cells are cultured and assembled into...
3D cultures of stem cells can preserve differentiation potential or increase the efficiency of methods that induce differentiation. Mouse bone marrow-derived stromal cells (BMSCs) were cultured in 3D as scaffold-free spheroids or “mesoid bodies” (MBs) and as aggregates on poly(lactic) acid mic...
Conclusion: A new 3D cell culture method that maximizes cell-to-cell interactions has been developed with a wide range of basic and translational applications.YoussefMorganYoussef, J.; Morgan, J. R. Self-Assembled Scaffold-Free Three-Dimensional Micro-Tissues for Tissue Repair. In Advances in ...
These optimizations enable the rapid formation of AT organoids and allow for the safe handling of scaffold-free 3D cell cultures. As a result, we present a straightforward, easily applicable 3D culture model for adipose tissue (AT) organoids that closely mimic the morphology and physiology of ...
We generate, for the first time, a scaffold free and stable self assembled 3 cell line co-culture 3D cardiac tissue model by assembling cardiomyocytes, endothelial cells and cardiac fibroblast cells via a rapid inter-cell click ligation process. We compare and analyze the function of the 3D ...
For in vitro co-culture, interaction between the stem cell and feeder cell as well as their spatial position are essential. To imitate the natural microenvironment, a 3D engineered scaffold for CD34cells co-culture was established via 3D bioprinting. Herein, the concentration of hydrogel and the ...
The cues include a biological growing template, spatial coculture, biomimetic radial flow, and circulation in a scaffold-free condition. The feasibility of recapitulating a multiscale physiological structural hierarchy, complex drug clearance, and zonal physiology from the cell to tissue level in long...
derived stem cells (hADSCs) are successfully cultured in this magnetic scaffold and the proliferation of hADSCs can be modulated by magnetically induced dynamic compression. This magnetic scaffold for dynamic 3D cell culture can be potentially utilized in cell biology and tissue engineering....