TaqPath DuraPlex 1-Step RT-qPCR Master Mix provides a full workday (over eight hours) of stability of completely assembled reactions (master mix, sample, and assay), helping enable the use of liquid handling and other automation equipment. This fast-cycling master mix can be used effectively ...
Meridian’s RT-qPCR Extraction Control Red uses artificial micelles of a known concentration that contain quantitative PCR positive control RNA sequence (with no known homology to any organism, so that it does not interfere with the detection of the sample target RNA). Th...
Meridian’s RT-qPCR Extraction Control Red uses artificial micelles of a known concentration that contain quantitative PCR positive control RNA sequence (with no known homology to any organism, so that it does not interfere with the detection of the sample target RNA). T...
TaqPath DuraPlex 1-Step RT-qPCR Master Mix enables a full workday (over eight hours) of stability of completely assembled reactions (master mix, sample, and assay), helping enable the use of liquid handling and other automation equipment. This fast-cycling master mix can be used effectively fo...
Applied Biosystems TaqPath DuraPlex 1-Step RT-qPCR Master Mix is a benchtop-stable, single-tube master mix optimized for rapid, sensitive, and reproducible detection of viral and bacterial pathogens even in the presence of PCR inhibitors. Supplier: Applied Biosystems™ A58669 ...
Full-length cDNA synthesis up to 12 kb More Information Applications Elimination of genomic DNA contamination cDNA synthesis for real-time RT-PCR (qPCR) Two-step real-time RT-PCR Choose the right PrimeScript kit for real-time RT-PCR:
T cell lines and primary CD4+ T cells. Late RT species and integrated viral DNA were quantified by qPCR and infectivity was measured by luciferase activity and p24 ELISA assay. Results were analyzed by the Student’st-test.ResultsUsing our integrase-LTR bait approach, we successfully identified ...
Because this gene was assayed on both QPCR plates for each patient sample, a direct comparison of CT values between plates could be made. The intra-specimen Pearson's correlation for RPLPO was 0.98. Figure 7 Comparison of paired raw C T values for reference normalization gene RPLPO for all...
The development and evaluation of this double-controlled BlueTYPE low-density RT-qPCR array for molecular serotyping of BTV positive samples in less than two hours, will be presented. 2. Material and methods 2.1. Primers and probes For each of the 24 classical BTV serotypes, the respective ...
71 DOI 10.1186/s12864-015-1224-y RESEARCH ARTICLE Open Access Tracking the best reference genes for RT-qPCR data normalization in filamentous fungi Agustina Llanos1,2,3,4, Jean Marie François1,2,3 and Jean-Luc Parrou1,2,3* Abstract Background: A critical step in the RT-qPCR workflow ...