Based on the direct PCR/qPCR technology platform, Foreivd Biotech brings an innovative and efficient "5H" screening solution - the Cell Direct RT qPCR kit. It can process cells in situ in culture plates to obtain RNA templates for reverse transcription, saving a lot of operation and time. I...
(with no known homology to any organism, so that it does not interfere with the detection of the sample target RNA). Their composition closely resembles a test sample and serves as a full-process quantitative PCR positive control, monitoring the success of an RT-qPCR assay f...
RT-qPCR has been used extensively to assess gene expression in entomological research17,18,19,20. Normalization of RT-qPCR data with reference genes is one of the most common strategies used to correct experimental errors introduced through the steps of RT-qPCR analysis; however, the choice of ...
Rapid detection of the SARS-CoV-2 omicron variants based on high-resolution melting curve analysis Article Open access 15 November 2024 Surveillance of SARS-CoV-2 lineage B.1.1.7 in Slovakia using a novel, multiplexed RT-qPCR assay Article Open access 14 October 2021 Introduction...
Full size image The expression data of the target genes given by FPKM (fragments per kilobase of exon model per million reads mapped) from the RNA-Seq was converted in log fold change (SD/LD) and compared to the RT-qPCR relative expression data, both in the 7th timepoint mature leaf sa...
Publicly available DLBCL gene expression data are available only in the form of microarray-based gene expression and not RT-qPCR gene expression. Thus, to use the LCOO classifier with microarray-based gene expression data, an equivalency study of the LCOO classifier with RT-qPCR–measured gene ...
SuperScript® III Platinum® One-Step Quantitative RT-PCR and OpenArray® technology on the QuantStudio™ 12K Flex system One-step reverse transcription (RT) quantitative polymerase chain reaction (qPCR) is a widely accepted technique that combines RT and real- time qPCR in the same reaction...
Table 1 MiRNAs, PCR primers and synthetic templates Full size table Figure 2 MiR-specific qPCR on synthetic templates with DNA primers. A The effect of primer concentration on Cq value of ssc-let-7d and ssc-miR-26a miR-specific qPCR assays. Real-time PCR assays were performed in parallel...
FR conditions was measured by RT‒qPCR. The expression in the roots under WL was standardized to be “1”. Data are presented as mean values +/− SD (n = 3,nrefers to biological replicates). Letters indicate significant differences between mean values (one-way ANOVA,P < 0.01...
To these samples were added a few drops of a MX solution to attain a suitable color intensity, which turned the samples violet, indicating that MX was in the free form. Addition of ZnCl2 at 8 mM to the samples rendered them orange, indicating a change of the indicator to its complexed ...