InniScript™ 荧光探针一步法qRT-PCR Mix是具有高灵敏度、高效合成能力和高扩增效率的一步法qRT-PCR试剂盒。本试剂盒以RNA为模板, 反向基因特异引物为反转录引物合成第一链cDNA;然后以合成的cDNA为模板,用正向、反向基因特异引物及荧光探针进行qPCR,在同一反应体系中一步完成从反转录到qPCR的全部反应。 产品特点...
Quantitative real-time PCR was performed in a 96 well format in the Bio-rad CFX96 Real Time System (BioRad Laboratories, USA). The real-time PCR mixtures consisted of 5 μL cDNA corresponding to ~600 ng total RNA, 0.1 uM of Real-Time primers or 0.2 nM of Invitrogen primer and 1× ...
Download: Download full-size image Fig. 5. Persistence of environmental DNA after heat-killing cultures shown as number of 18S V9 template copies measured by qRT-PCR, relative to untreated numbers at time zero (circles). Squares (right Y-axis) show the calculated number of dead BC sequences...
Here, we describe development of a qRT–PCR test that predicts tissue of origin of FFPE tumor samples. Materials and methods Samples Tumor samples were obtained from several sources (Beilinson Hospital, Rabin Medical Center, Petah- Tikva, Israel; Sheba Medical Center, Tel-Hashomer, Israel; Tel ...
Download: Download full-size image Fig. 2. Representative qRT-PCR amplification plots for the SARS-CoV-2 ORF1ab (FAM) and N (HEX) genes for a nasopharyngeal swab sample subjected to the standard RNA extraction, heat-inactivation (H), and heat-inactivation and pelleting (HC). Amplification ...
Full size image 2. Validation phase. SncRNAs candidates were tested by qRT-PCR in an independent cohort of 102 patients at baseline. To determine the gene stability during follow-up, the analysis was extended T1 (1 month after treatment) and T2 (6 month after treatment). After gene stabilit...
Cyperus esculentus; tuber; reference gene; qRT-PCR; gene expression; normalization; RankAggreg1. Introduction To pursue a better quality of life and a healthier diet for humans, many economically important crops have been intensively studied. Meanwhile, an increasing number of agricultural sources of...
The qRT-PCR expression results provided in the form of Ct values, were used for the calculation of statistical significance through NormFinder software (https://moma.dk/normfinder-software accessed on 12 August 2022). As NormFinder ranks the stability of the tested genes independent to each other...