Here, we demonstrate that seed mice containing the RMCE landing pad can be developed rapidly by using the CRISPR/Cas9 system. One of the CRISPR targets tested in this study enabled the insertion of sequences precisely at the original ROSA26 provirus integration site. We anticipate that using a...
Platt R, Chen S, Zhou Y, Yim M, Swiech L, Kempton H,et al. CRISPR-Cas9 knockin mice for genome editing and cancer modeling. Cell. 2014;159:440-55pubmedpublisher product information Catalog Number : 61408 Product Name : LSL-Cas9-Rosa26TV ...
direct efficient, liver-specific expression in transgenic mice. Genes Dev 1(3): 268-276. 备注 - 上一篇: SD-GFAPem35Cin(Cre)/Nju模型 下一篇: SD-H11em1Cin(LSL-tdTomato)/Nju模型 检测流程 填写并提交定制服务需求表 技术评估和方案讨论 对选定的试验方法进行报价 合同签定与付款 按期交付...
and consequently dCas9-VPR expression activation, in a tissue specific manner, Rosa26-LSL-dCas9-VPR mice were either injected with a preferentially liver transducing AAV8 containing theCregene under the control of a liver specificLP1promoter (AAV8-Cre) or AAV8 carrying U6 promoter driven guide ...
TransgeneZygotesGenetically modified (GM) mice are widely used in biomedical research because they can address complex questions in an in-vivo setting that could not otherwise be addressed in-vitro. Microinjection of zygotes remains the most common technique to generate GM animals to date. Here, ...
LABORATORY miceANIMAL disease modelsGENE expressionTransgenic animals with increased or abrogated target gene expression are powerful tools for drug discovery research. Here, we developed a CRISPR-based Rosa26-LSL-dCas9-VPR mouse model for targeted induction of endogenous gene expression using different...
Gao used α-actin protein regulators to drive the expression of IGF-1 in mouse myoblasts and obtained transgenic mice with muscle hypertrophy [45]. Zou used the skeletal muscle-specific myosin light chain enhancer to upregulate the expression of IGF-1 specifically in skeletal muscle and obtained ...