with the application of CRISPR/Cas9-based knockin technology, large DNA fragments can be accurately introduced into the ROSA26 locus quickly and efficiently, with turnaround times as short as 3 months. We guarantee delivery of a minimum of 2 founders or 3 F1 animals for ROSA26 knockin with in...
Price:$1,451.00 Catalog#: SH079 SH075 SH054 SH050 SH350 SH351 SH352 Size:Cas9 ROSA26 knockin donor clone Qty: Manual: View your cart Click here for the Homepage of Safe Harbor Gene Knock-in Introduction TALEN Kits CRISPR kits Services ...
Platt R, Chen S, Zhou Y, Yim M, Swiech L, Kempton H,et al. CRISPR-Cas9 knockin mice for genome editing and cancer modeling. Cell. 2014;159:440-55pubmedpublisher product information Catalog Number : 61408 Product Name : LSL-Cas9-Rosa26TV ...
ROSA26-specific TALENs or CRISPR-Cas9 system can generate a DNA DSB in ROSA26 in mouse genome, stimulating natural DNA repair mechanism. In the presence of ROSA26 ORF knockin clones, homologous recombination HR happens and integrantes DNA fragment from ORF knockin clone into the safe harbor locus...
Cas9/AAV9-Mediated Somatic Mutagenesis Uncovered the Cell-Autonomous Role of Sarcoplasmic/Endoplasmic Reticulum Calcium ATPase 2 in Murine Cardiomyocyte Maturation miRNA‐200c‐3p promotes endothelial to mesenchymal transition and neointimal hyperplasia in artery bypass grafts ...
These fusion proteins can recognize and bind chromosome target sequences specifically and execute their gene-editing functions, such as gene knockout, knockin (with donor plasmid), modification, activation, repression and more. Introduction to CRISPR-Cas9 In the CRISPR-Cas9 system, the complex of a...
dCas9-VPR expression was restricted to liver, with absent or only minor but not statistically significant increases in all other investigated tissues, including heart, lung, kidney, and spleen (Fig.1D). These results show that Rosa26-LSL-dCas9-VPR mice in combination with AAV8 and LP1 promote...
Moreover, in AAV8-Cre treated mice, dCas9-VPR expression was restricted to liver, with absent or only minor but not statistically significant increases in all other investigated tissues, including heart, lung, kidney, and spleen (Fig. 1D). These results show that Rosa26-LSL-dCas9-VPR ...
Briefly, sgRNA targeting rbRosa26 is co-introduced to pronuclear stage rabbit embryos with Cas9 mRNA and the donor DNAs. The donor DNA template (Fig. 2A) consists of a splice acceptor (SA), a promoterless tdTomato gene, and an inverted EGFP (iEGFP) flanked by homologous arms. To ...
5)细胞转染:将所述crispr/cas9表达质粒px458-rosa26与供体载体共转染进入小鼠es细胞; 6)用流式细胞技术先通过绿色荧光分选出成功转染质粒的细胞进行培养7天后,通过红色荧光进行单细胞分选,经培养获得定点整合外源基因的es单克隆细胞系。 优选地,所述crispr表达质粒的grna的oligo序列如seqidno.2和seqidno.3所示。