(N-cadherin),Notch1,Hes1蛋白表达.[结果]乳腺癌MCF-7,MDA-MB-231,MDA-MB-361细胞中ROR1-AS1水平明显高于正常乳腺上皮MCF10A细胞(P<0.05).与对照组,sh-NC组比较,sh-ROR1-AS1组乳腺癌MCF-7细胞存活率降低(100.00%±9.82%,99.74%±12.05%,58.91%±6.33%),细胞凋亡率升高(4.81%±0.26%,4.93%±0.34%,...
目的探讨长链非编码RNA(lncRNA)ROR1-AS1对骨肉瘤(OS)侵袭能力的影响.方法RT-PCR检测成骨细胞和OS细胞株中lncRNA ROR1-AS1和ROR1的表达;行细胞转染,观察细胞生长情况,再过表达ROR1,Transwell和划痕实验检测细胞侵袭能力;Western-blot检测上皮间质转化相关分子标志物E-caderin,N-caderin,abcam的表达.结果与成骨细...
ROR1-AS1 is a cancer-associated lncRNA, and studies have found that it is either over- or underexpressed in multiple malignancies, including liver cancer, colon cancer, osteosarcoma, glioma, cervical cancer, bladder cancer, lung adenocarcinoma, and mantle cell lymphoma. Furthermore, it has been...
Firstly, we assessed cell viability, proliferation, invasion, and migration using three cell lines (HuCCT-1, QBC399, and RBE) to explore the biological characteristics of ROR1-AS1 in CCA. Secondly, to determine the in vivo effect of ROR1-AS1 on tumor growth, ROR1-AS1 knockdown (KD) Hu...
凋亡该研究旨在探讨ROR1-AS1对神经母细胞瘤细胞SK-N-SH增殖,迁移,侵袭和凋亡的影响及作用机制.收集67例神经母细胞瘤组织和瘤旁组织,实时荧光定量PCR(RT-qPCR)检测组织中ROR1-AS1和miR-758-3p的表达情况.转染ROR1-AS1小干扰RNA,miR-758-3p模拟物或共转染ROR1-AS1小干扰RNA与miR-758-3p抑制剂... 查看全部>...
This study aimed to determine the expression and clinical significance of lncRNA ROR1 antisense RNA 1 (ROR1-AS1) from patients with bladder cancer, and to explore the potential role and mechanism underlying ROR1-AS1-related cancer progression. Methods Real time quantitative PCR (RT-qPCR) was ...
Long noncoding RNA ROR1-AS1 induces tumor metastasis and epithelial-mesenchymal transition by sponging miR-375 in nasopharyngeal carcinomaHOU, J.YAN, J.REN, X.-Y.ZHU, K.DU, X.-Y.LI, J.-J.XU, M.European Review for Medical & Pharmacological Sciences...
ROR1-AS1可靶向调控miR-504.与si-ROR1-AS1+anti-miR-NC组比较,si-ROR1-AS1+anti-miR-504组细胞克隆形成数,细胞存活率,迁移细胞数,侵袭细胞数及MMP-2,MMP-9水平升高(t=7.064,7.012,6.746,10.222,7.213,7.982,P均<0.001),p21,E-cadherin蛋白水平下降(t=4.841,7.120,P均<0.001).结论干扰ROR1-AS1表达可...