Furthermore, Kaplan-Meier curve analysis revealed that increased EZR-AS1 expression in patients with BC contributes to poor prognosis. Cell counting kit-8 and fluorescence-activated cell sorting experiments indicated that EZR-AS1 knockdown significantly suppressed the proliferation and cell cycle progression...
EZR-AS1 was knocked down in two lung ADC cell lines using small interfering RNA specific for EZR-AS1 (siEZR-AS1). Proliferation, migration, and apoptosis of EZR-AS1-knockdown cells were assessed using the CCK-8 viability assay, flow cytometry, or wound healing experiments. The levels of ...
HCT116 and HT29 cells were then transfected with EZR-AS1 shRNA (sh-EZR-AS1) to silence lncRNA EZR-AS1 (P<0.01). When compared with the Control, after transfection of SH-EZR-AS1, E-cadherin was up-regulated, Vimentin was down-regulated, the apoptosis rate was increased, the cell ...
EZR-AS1、肺癌、增殖、迁移侵袭目的探索氯普鲁卡因对肺癌细胞增殖和迁移侵袭的影响及其分子机制.方法使用1 mmol/L,1.5 mmol/L,2 mmol/L浓度的氯普鲁卡因处理肺癌A549细胞,噻唑蓝(MTT)检测细胞增殖,Transwell检测细胞迁移侵袭,蛋白质印迹法(Western blot)检测细胞周期蛋白D1(CyclinD1),P21,基质金属蛋白酶-2(MMP-2)...