we could use single-nucleotide polymorphisms (SNPs) to infer the complete SLR-X and SLR-Y haplotypes in the sex-linked region (Fig.1b). The X and Y haplotypes’ telomeric repeat regions were assembled into two contigs, of sizes 104 kb and 141 kb, both well supported by mapped raw ...
Early life exposure to adverse environments affects cardiovascular and metabolic systems in the offspring. These programmed effects are transmissible to a second generation through both male and female lines, suggesting germline transmission. We have pre
Dried DNA pellet was dissolved in TE buffer. Quantitative polymerase chain reaction using group-specific PCR primers were used to quantify relative abundance of different groups to the total gut microbiota as reported previously38, 46, 47 and the sequences of PCR primers are provided in ...
The epidemiological profiles of vector-borne diseases, such as malaria, are strongly associated with landscape components. The reduction of malaria burden in endemic and epidemic regions mainly depends on knowledge of the malaria-transmitting mosquito species, populations and behavioural characteristics, as...
mouse intraperitoneal PMT administration retarded the growth of xenografted tumors derived from human ovarian cancer SKOV3 Her2+ cells. Conclusions/Significance We conclude that PMT is usually active in suppression of cell proliferation and tumor growth. Introduction There was a time when most medicinal...
Cabbage Fusarium wilt (CFW) is a devastating disease caused by the soil-borne fungus Fusarium oxysporum f. sp. conglutinans (Foc). One of the optimal measures for managing CFW is the employment of tolerant/resistant cabbage varieties. However, the interp
The mitochondria were then purified on discontinuous sucrose gradients consisting of 1.6 M (4 ml) and 1.15 M (7 ml) sucrose in SEM buffer in 13 PET centrifuge tubes. The crude mitochondrial suspensions were layered onto the sucrose gradients and centrifuged at 22,500 rpm for 1 h at 4°C ...
Role of Glycine N-Methyltransferase in the Regulation of T-Cell Responses in Experimental Autoimmune Encephalomyelitis Chung-Hsien Li,1,2 Ming-Hong Lin,3 Shih-Han Chu,1,2 Pang-Hsien Tu,4 Cheng-Chieh Fang,1,2 Chia-Hung Yen,2,5 Peir-In Liang,6 Jason C Huang,7 Yu-Chia Su,8 Huey-Kang...
regulations set forth by the IACUC. For genotyping, genomic DNA was isolated from either clipped ears by incubating the tissues overnight at 55 °C in the lysis buffer (0.1 M Tris-Cl, pH 8.8, 5 mM EDTA, pH 8.0, 0.2% SDS, 0.2 M NaCl, 20 μg/ml Proteinase K). The...
coli RNase H1 at the (5')RNA-DNA(3') junction in advance or not. When this substrate was cleaved by a mixture of E. coli RNases H1 and H2, the ribonucleotide was removed from the substrate. We propose that RNase H1 is involved in the excision of single ribonucleotides misincorporated...