Sample was centrifuged at 7000 g for 3 min and aqueous phase was collected and a second round of phenol/chloroform/isoamyl extraction was performed. DNA from the clear aqueous phase was precipitated using sodium acetate and isopropanol. Dried DNA pellet was dissolved in TE buffer. ...
Publication » 1308 A ROLE FOR DNA REPAIR PROTEIN KINASE (DNA-PK) IN THE PROGRESSION OF SIMPLE STEATOSIS TO STEATOHEPATITIS (NASH)?.doi:10.1016/S0168-8278(13)61309-4Whitehead, A.Patman, G.L.Cornell, L.Televantou, D.Curtin, N.
Purified RNA was eluted in TE buffer (Tris-HCl 10 mM, pH 7.5, EDTA 1 mM). RNA concentrations were determined using an ND-1000 NanoDrop spectrophotometer (Thermo Scientific) or by Qubit RNA HS fluorometric assay (Thermo Fisher, Q32855). Total RNA samples were collected in 2.0 ml ...
Cabbage Fusarium wilt (CFW) is a devastating disease caused by the soil-borne fungus Fusarium oxysporum f. sp. conglutinans (Foc). One of the optimal measures for managing CFW is the employment of tolerant/resistant cabbage varieties. However, the interp
mouse intraperitoneal PMT administration retarded the growth of xenografted tumors derived from human ovarian cancer SKOV3 Her2+ cells. Conclusions/Significance We conclude that PMT is usually active in suppression of cell proliferation and tumor growth. Introduction There was a time when most medicinal...
Here, we demonstrate that moderate mitochondrial superoxide and hydrogen peroxide production oxidates KEAP1, thus breaking the interaction between this protein and PGAM5, leading to the inhibition of its proteasomal degradation. Accumulated PGAM5 interferes with the processing of the PINK1 in the ...
Oncogene HCCR-1 functions as a negative regulator of the p53 and contributes to tumorigenesis of various human tissues. However, it is unknown how HCCR-1 contributes to the cellular and biochemical mechanisms of human tumorigenesis. In this study, we sho
Protein-DNA binding was measured, briefly, as follows, using the LightShift™ Chemiluminescent EMSA Kit (Pierce, Rockford, IL): equal amounts (10 μg) of nuclear extract protein were used in each reaction, extracts were preincubated with a binding buffer containing 50% glycerol, 50 ng/μl...
Nucleosome positioning can be assayed by limited enzymatic digestion of chromatin using micrococcal nuclease (MNase) or other enzymes8,9,10,11, which are partially occluded from nucleosome-wrapped DNA and preferentially digest inter-nucleosomal regions6,12. A strength of this strategy is that the ent...
of FN and PE in an integrin b1-dependent manner; (3) a dominant-negative mutant lacking the PINCH-binding N-terminus of ILK (ILK-C) prevents costamere association of ILK and alpha-parvin, but not PINCH1; (4) FN- and PE-induced hypertrophy, measured by increased protein/DNA ratio, ...