A Tris–EDTA (TE) buffer-based boiling method of DNA extraction developed earlier by us was employed and explored for its suitability in the detection of Wuchereria bancrofti DNA in pools of Culex quinquefasciatus mosquitoes in real-time PCR assay. In this preliminary study, 1,000 laboratory-...
Life may be expressed as the flow of electrons, protons, and other ions, resulting in large potential difference. It is also highly photo-sensitive, as a large proportion of the redox capable molecules it relies on are chromophoric. It is thus suggestive
This hampers the use of this technology to rapidly obtain critical sequence information in order to be able to develop a specific qPCRdetection method. Methods Data generated by NGS were exploited using a simple BLAST approach. A TaqMan® qPCR method was developed and tested on isolated ...
circles to characterize the covalent topoisomerase-DNA complex Armêl Millet, François Strauss & Emmanuelle Delagoutte The enzymatic DNA relaxation requires the DNA to be transiently nicked and rejoined, the covalent topoisomerase-DNA complex being a key intermediate of the nicking-joining ...
Phusion HighFidelity DNA polymerase (Thermo scientific) XhoI (Thermo Scientific) NcoI (Thermo Scientific) GelRed (Nucleic Acid Gel, Biotium) 1X TBE buffer (Tris-HCL/Boric Acid/EDTA) TE buffer [10 mM Tris-HCl, pH 8.0 and 1 mM (ethylenedinitrilo)tetraacetic acid (EDTA)] Sodium acetate (3...
Recombinant protein production and purification of large protein complexes in eukaryotes requires efficient methods to generate multi-gene expression constructs, where each individual gene is under the control of its own promoter and terminator. Current methods are based either on serial rounds of combinat...
Several eukaryotic DNA viruses maintain their genomes as extrachromosomal multicopy nuclear episomes in proliferating host cells. Such episomal maintenance is characteristic of latent infection of the Bovine papillomavirus type 1 (BPV-1), Epstein-Barr Virus (EBV) as well as for Kaposi sarcoma associated...
The collected samples were immediately put in liquid nitrogen, transported in dry ice and stored at −20 °C, then, soil pH was determined in a 1:1 (wt/wt) soil–H2O slurry prior to DNA extraction. 2.2. DNA Extraction and Whole Genome Shotgun Sequencing...
Being an essential element, P is involved in the synthesis of DNA, RNA and membrane proteins and lipids. It acts as a signaling molecule and is associated with cellular phosphorylation events [7]. Plants absorb P from the soil mainly in the form of soluble inorganic P. In soil, P ...
Microsatellites or single sequence repeats (SSRs) are a powerful choice of marker in the study of Phytophthora population biology, epidemiology, ecology, genetics and evolution. A strategy was tested in which the publicly available unigene datasets extra