A Tris–EDTA (TE) buffer-based boiling method of DNA extraction developed earlier by us was employed and explored for its suitability in the detection of Wuchereria bancrofti DNA in pools of Culex quinquefasciatus mosquitoes in real-time PCR assay. In this preliminary study, 1,000 laboratory-...
Depending on the excitation process and the type of metal involved, when the absorbed energy is released, it can damage the DNA and/or modify the protein, promote lipid peroxidation and destroy the cell microenvironment, hence causing cell death. This method is promising in fighting cancer cells...
This hampers the use of this technology to rapidly obtain critical sequence information in order to be able to develop a specific qPCRdetection method. Methods Data generated by NGS were exploited using a simple BLAST approach. A TaqMan® qPCR method was developed and tested on isolated ...
circles to characterize the covalent topoisomerase-DNA complex Armêl Millet, François Strauss & Emmanuelle Delagoutte The enzymatic DNA relaxation requires the DNA to be transiently nicked and rejoined, the covalent topoisomerase-DNA complex being a key intermediate of the nicking-joining ...
PCR reac- tions comprised: 0.5 µM of primers, 0.02 U/µl of Phu- sion High-Fidelity DNA Polymerase (Thermo Scientific, Copenhagen, Denmark), 2 mM of dNTP mix, 1 × Phu- sion HF Buffer (Thermo Scientific, Copenhagen, Den- mark) and 10 ng cDNA in a total ...
(20 mM Tris-HCl, pH 8; 0.5% NP-40; 0.5% sodium deoxycholate; 1 mM EDTA; 250 mM LiCl) and TE Buffer (10 mM Tris-HCl, pH 8; 1 mM EDTA), and eluted in Elution Buffer (50 mM Tris-HCl, pH 8; 1 mM EDTA; 1% SDS) followed by TE containing 0.67% SDS for 10...
Phusion HighFidelity DNA polymerase (Thermo scientific) XhoI (Thermo Scientific) NcoI (Thermo Scientific) GelRed (Nucleic Acid Gel, Biotium) 1X TBE buffer (Tris-HCL/Boric Acid/EDTA) TE buffer [10 mM Tris-HCl, pH 8.0 and 1 mM (ethylenedinitrilo)tetraacetic acid (EDTA)] Sodium acetate (3...
Recombinant protein production and purification of large protein complexes in eukaryotes requires efficient methods to generate multi-gene expression constructs, where each individual gene is under the control of its own promoter and terminator. Current methods are based either on serial rounds of combinat...
It is thought to be inactive in transcription, has a low gene density, and is replicated late in the S-phase5,6. It can be further classified into constitutive heterochromatin, which is composed of repetitive sequences of DNA known as satellite repeats and is associated mostly with peri- ...
Theobroma cacao L. cultivar Matina 1-6 belongs to the most cultivated cacao type. The availability of its genome sequence and methods for identifying genes responsible for important cacao traits will aid cacao researchers and breeders. We describe the se