cDNA of each strain was synthesized using M-MLV reverse transcriptase (Promega Corp., San Luis Obispo, CA, USA), dNTPs, and random primers (Takara, Shiga, Japan) according to the manufacturer’s instructions, and total RNA was used as the template, RNA were extracted from three paralleled ...
1. Can methylation of nucleotides play a role in DNA replication? 2. If so, what role does it play? a. Removal of methyl groups from nucleotides is concurrent with the conversion of dNTPs to dNMPs during DNA synthesis. b. Lack of methylation on the synthe ...
Briefly, MHC loci were amplified separately by PCR of genomic DNA in 30 μl reaction volume consisting of 20 ng DNA, 1 mm PCR buffer, 1.5 mm MgCl2, 0.2 nmol dNTPs, 0.3 μm of each primer, 4 mm Tetramethyl ammonium chloride and 0.2 units of Taq-Polymerase. PCR cycling...
1. Can methylation of nucleotides play a role in DNA replication? 2. If so, what role does it play? a. Removal of methyl groups from nucleotides is concurrent with the conversion of dNTPs to dNMPs d...
Influenza infection increases the incidence of myocardial infarction but the reason is unknown. Platelets mediate vascular occlusion through thrombotic functions but are also recognized to have immunomodulatory activity. To determine if platelet processe
Nucleoside diphosphate kinase (NDK), a house-keeping enzyme that plays a critical role in the synthesis of nucleic acids precursors, i.e. NTPs and dNTPs, is absent in all the Mollicutes genomes sequenced to date. Therefore, it would be of interest to know how Mollicutes synthesize dNTPs/NT...
As shown by scanning electron microscopy (SEM), the produced Mg-PPi microparticles consisted of intersecting disks or their segments. They were morphologically similar but simpler than DNA-containing MPs generated in PCR. The incorporation of dNTPs, primers, or dsDNA into Mg-pyrophosphate particles ...
RT-PCR and Western blotting was employed to investigate the expression of TLRs 1–10 in primary IPE and RPE. Secretion of IL-8 or MCP-1 following treatment with PAMPs was measured by ELISA. The role of TLR2, TLR3 and TLR4 in mediating an inflammatory response was investigated using pharmac...
What do dNTPs provide during DNA replication? How are they different from dNMPs? What is the role of RNA and how does it differ from DNA? Why is it important that DNA replication not be 100% accurate? (a) What are the different enzymes used in DNA replication? (b) What are their fu...
A 20 μl reverse transcription reaction mixture containing 1 μg of total RNA, 1× reverse transcription buffer (10 mM Tris-HCl, pH 9.0, 50 mM KCl, 0.1% Triton X-100), 1 mM deoxynu- cleoside triphosphates (dNTPs) 0.5 unit of RNase inhibitor, 0.5 μg of oligo (dT)15, and 15 ...