Ribonuclease P (RNase P) is the onlytrans-acting RNA enzyme found in all kingdoms of life, a multiturnover ribozyme that edits the 5′ ends of precursor tRNA (pre-tRNA) and other RNAs10,30,31. Human RNase P processes the metastasis-associated lung adenocarcinoma transcript 1 (MALAT-1), ...
Ribonuclease P (RNase P) is the onlytrans-acting RNA enzyme found in all kingdoms of life, a multiturnover ribozyme that edits the 5′ ends of precursor tRNA (pre-tRNA) and other RNAs10,30,31. Human RNase P processes the metastasis-associated lung adenocarcinoma transcript 1 (MALAT-1), ...
Intriguingly, we found a positive correlation between probe uptake and RNase L expression levels (Fig. 6B). MCF-7 cells, which express the highest levels of RNase L, showed the greatest probe uptake, followed by HEK-293 cells, with MDA-MB-231 cells showing the lowest uptake. This ...
2b). The surface electrostatic potential of Mtb-RNase J was evenly distributed, except that the RNA binding pocket was full of positive charges (Fig. 2c). Fig. 2: The overall structure of Mtb-RNase J. a Domain structure of Mtb-RNase J. The β-lactamase domain, β-CASP domain, and ...
Zhang J, Dyer KD, Rosenberg HF: Evolution of the rodent eosinophil-associated RNase gene family by rapid gene sorting and positive selection. Proc Natl Acad Sci (USA). 2000, 97: 4701-4706. 10.1073/pnas.080071397. Article PubMed Central CAS Google Scholar Harder J, Schröder JM: RNase ...
For each step, the remaining read numbers were calculated (Additional file1: Table S1). Overall mappability of these reads was 29.2%. Redundancy (redundant mapped reads / mapped reads) was also calculated for each library. The number of mapped reads and the redundancy have a strong positive co...
*, p < 0.05; **, p < 0.01; ***, p < 0.001; ***, p < 0.0001. The data are presented as means ± S.D. RNASET2 regulates the amount of mitochondrion-associated cytosolic rRNAs in vivo and has a positive effect on nuclear transcription To examine whether the in organello findings...
Right: Quantification of DNA damage-positive cells. The graphs indicate the percentage of nuclei containing more than two foci positive for both γ-H2AX and p-SQ/TQ. Error bars indicate the mean ± SD of biological triplicates. One-way ANOVA coupled with Dunnett’s multiple comparisons ...
Moreover, a pSGD cassette carrying a gRNA targeting the beginning part of the Venus transgene was used as the electrotransfection positive control by which the Venus knock-out could also be mediated through NHEJ events1. Similarly, long-term selection of electrotransfected cells with the pSGD ...
Northern blot analysis of total RNA from human cell lines and Poly A containing RNA from human tissues using the 1.7 kb full length probe or a 332-nucleotide probe that contained the 5′ UTR and coding region of human RNase H cDNA revealed two strongly positive bands with approximately 1.2 ...