课程包括7个模块:1) Introduction to Genomics Analysis; 2) Data Preprocessing and Quality Control; 3) Comparative Experiments based on Microarrays and Linear Models (LM); 4) Comparative Experiments based on RNA-sequencing and Generalized Linear Models (GLM); 5) Comparative Experiments based on Differ...
文章标题:A single-cell analysis reveals tumor heterogeneity and immune environment of acral melanoma. 01研究背景 肢端黑色素瘤(AM)是一种发生在肢端皮肤的黑色素瘤(CM)亚型,最近的研究显示,免疫疗法对黑色素瘤的治疗响应率可达到38%,但对AM的响应率只有16.6%。该研究旨在通过单细胞测序,从肿瘤异质性和免疫环...
2. Vieth, B., et al.,A systematic evaluation of single cell RNA-seq analysis pipelines.Nat ...
(Gene Set Enrichment Analysis, GSEA) 一般差异富集分析(GO和KEGG)需设置差异基因筛选阈值,侧重关注少数显著上调或下调的基因,容易遗漏部分差异表达不显著却有重要生物学意义的基因,忽略部分基因间调控关系,对筛选出的单个基因难以研究其功能和生物学意义,且有时会出现我们所关注的关键基因或通路差异表达并不显著的情况。
iii) pathway analysis, iv) fusion gene detection v) expressed variant calling. 参考 Google search:RNA-seq library construction The impact of RNA sequence library construction protocols on transcriptomic profiling of leukemia Introduction to RNA-Seq for Researchers- YouTube - 整个过程讲得比较全面 ...
Comparative Analysis of Single-Cell RNA Sequencing Methods The Technology and Biology of Single-Cell ...
read counts是测序之后每个基因测到的reads数量，可用于做差异分析，因为做差异分析的软件要求输入原始reads...
ASAP（Automated Single-cell Analysis Pipeline) ：是一款单细胞分析的交互式网络平台。从基因表达矩阵开始...
第二篇文献：Single-nucleus transcriptome analysis reveals dysregulation of angiogenic endothelial cells ...
课程包括7个模块:1) Introduction to Genomics Analysis; 2) Data Preprocessing and Quality Control; 3) Comparative Experiments based on Microarrays and Linear Models (LM); 4) Comparative Experiments based on RNA-sequencing and Generalized Linear Models (GLM); 5) Comparative Experiments based on ...