In an effort to uncover the transcripts and miRNA networks involved in plant height, the transcriptome and small RNA sequencing were performed based on dwarf mutant Ari1327 (A1), tall-culm mutant Ari3697 (A3) and wild type Ari971 (A9) in Gossypium hirsutum. The stem apexes of wild-type ...
本研究使用籼 稻油占 8 号和广西普通野生稻构建的 CSSL 群体以及 现代分子生物学技术,对广西普通野生稻进行研究, 通过二代测序(next generation sequencing, NGS)和 混池分析(bulk segregation analysis, BSA)方法,鉴 定广西普通野生稻基因组中与株高相关的 QTL,进 而筛选出在株高调控中可能起到关键作用的候选...
Leaf and root tissues were collected at the panicle initiation stage of the plant for transcriptome analysis. Figure 3. Tissue-specific differential expression of genes on direct-sowing. (A) Leaf, and (B) Root. Change in expression (Up = up-regulation, or Dn = down-...
RNA-seq analysis Total RNA was extracted with the Plant RNeasy mini kit (Qiagen, Germany) and DNase treated on column according to the manufacturer’s protocol. A Truseq RNA library was constructed according to the manufacturer’s protocol (Illumina, USA). Raw sequencing reads were generated by...
3.6. RNA sequence analysis and qRTPCR validation A total of 264.3 million reads were obtained from six samples through RNA sequencing, resulting in an average of 44.05 million reads (Table S3). The total number of reads that aligned to the reference genome (Gossypium hirsutum L. acc. TM-1)...
3 Chromatograms of the DNA sequencing of CRE sites 菌(Botrytis cinerea Pers), 禾谷镰孢菌(Fusarium graminearum), 立枯丝核菌(Rhizoctonia solani Kühn)进行平板抗菌实验,表 5 为每隔 24 h 测量的 真菌生长圈直径,结果表明 B.pumilus LG3145 对 6 种植物致病菌都表现出较强的抑制性,在 LG3145 组 中...
Since the development of RNA sequencing [1,2], our understanding of the transcriptome and its regulation has grown substantially [3]. By expanding the context of transcriptome analysis, RNA sequencing allows for an improved understanding of the effects of cellular regulation [4,5], the environment...
RNA-sequencing (RNA-seq) has a wide variety of applications, but no single analysis pipeline can be used in all cases. We review all of the major steps in RNA-seq data analysis, including experimental design, quality control, read alignment, quantificati
Analysis of quantitative RNA sequencing data, focusing on differential expression of mRNA and repeat RNA. Supplementary Data 4 Upregulated pathways revealed by GSEA between WT and FTO-transgenic rice shoots and roots. Supplementary Data 5 Unprocessed gel for Supplementary Fig. 1c. Supplementary Data ...
analysis for species without a reference transcriptome. By coupling ultra-fast read mapping algorithms with high-resolution ortholog databases through a user-friendly web interface, ExpressAnalyst allows researchers to obtain global expression profiles and gene-level insights from raw RNA-sequencing reads ...