Over the past decade, RNA sequencing (RNA-seq) has become an indispensable tool for transcriptome-wide analysis of differential gene expression and differential splicing of mRNAs. However, as next-generation sequencing technologies have developed, so too
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CNKI南京航空航天大学学报(英文版)ZHANG L, LIU X J. A comprehensive review on RNA-seq data analysis[J]. Transactions of Nanjing University of Aeronautics and Astronautics, 2016, 33(3): 339-361.
RNA-sequencing (RNA-seq) has a wide variety of applications, but no single analysis pipeline can be used in all cases. We review all of the major steps in RNA-seq data analysis, including experimental design, quality control, read alignment, quantification of gene and transcript levels, visual...
Transcriptome sequencing (RNA-Seq) has become the assay of choice for high-throughput studies of gene expression. However, as is the case with microarrays, major technology-related artifacts and biases affect the resulting expression measures. Normalizat
Single-cell RNA-seq highlights intra-tumoral heterogeneity and malignant progression in pancreatic ductal adenocarcinoma. Cell Res. 29, 725–738, https://doi.org/10.1038/s41422-019-0195-y (2019). Article CAS PubMed PubMed Central Google Scholar Elyada, E. et al. Cross-species single-cell ...
RNA-seq文章细胞组分分离 您想要研究单细胞RNA,我们提供单细胞RNA提取试剂盒 最近生物人的朋友圈被“北大汤富酬”这五个字刷屏了,这位学者大牛连发9篇高水平文章,刚刚发表的一篇题为“Dissecting the global dynamic m... 10月17日 阅读 被查看3,365 次 发表评论 ...
scRNA-seq provides new technical means for further development of tumor research and is expected to make significant breakthroughs in this field. This review focuses on the principles of scRNA-seq, with an emphasis on the application of scRNA-seq in tumor heterogeneity, pathogenesis, and treatment....
Article | 01 December 2022 Quantification of extracellular proteins, protein complexes and mRNAs in single cells by proximity sequencing This work presents Prox-seq that couples sequencing and proximity ligation assay to simultaneously measure extracellular proteins, protein–protein interactions and mRNA ...
This Review summarizes how next-generation RNA sequencing (RNA-seq) has become a primary technology to study host–microbe interactions with high resolution, improving our understanding of the physiological consequences and the mechanisms at play. We illustrate how the discriminatory power and sensitivity...