# 下载流程git clone https://github.com/nf-core/rnaseq.git 下载本地化镜像,并将设置镜像地址 # 在相同目录下,需在联网环境下 nf-core download --outdirsingularity/ --parallel-downloads 10 --container-system singularity rnaseq # 会跳出几个选项,选择默认的就行 # 设置镜像地址 export NXF_SINGULARI...
此次示范,我们选择的是最常用的rnaseq流程。 所涵盖的部分流程 2. 如何下载Nextflow和nf-core? 此篇教学文档,主要针对于需要在服务器上(特别是计算节点)运行nf-core/rnaseq流程的人,也就是官方流程中所述的Running Offline(离线运行)。 官方的安装方法文档:https://nf-co.re/usage/installation 离线运行所需的...
1. 下载安装包: nextflow可以通过bioconda安装,所以这次我们只需要下载nf-core/rnaseq的安装包即可: 1 2 3 4 mamba create -n nextflow nextflow nf-core conda activate nextflow nf-core download nf-core/rnaseq tar-zxvf nf-core-rnaseq-3.4.tar.gz 此时我们可以通过nextflow运行这个安装包: 1 2 3 4 5...
working with gene panels or single genes (e.g., NF1) in the nf-core/rnaseq pipeline #1472 openedDec 18, 2024byhuma1995 1 Unify SummarizedExperiment outputenhancement #1465 openedDec 10, 2024bygrst 2 ERROR Not able to pull image of ('biocontainers/bioconductor-tximeta:1.20.1--r43hdfd...
RNA sequencing analysis pipeline using STAR, RSEM, HISAT2 or Salmon with gene/isoform counts and extensive quality control. - rnaseq/workflows/rnaseq.nf at 3.10.1 · nf-core/rnaseq
癫痫小鼠和过表达SerpinA3N的小鼠海马中Tnf-α、Il-1β、Il-18、Il-6和Nf-κb的表达水平升高,而SerpinA3N敲低逆转了这些变化,表明KA和SerpinA3N可单独诱导炎症因子的上调。此外,SerpinA3N过表达加重了KA注射后35 d小鼠中这些mrna的上调,SerpinA3N敲低逆转了这些变化。
DAO and endotoxin in rats were detected by ELISA method; the differentially expressed genes were detected in ileal tissues of rats in each group by RNA-seq sequencing, so as to screen the core genes, biological functions and signaling pathways of the QJH...
e, 增强子被归类为ATAC-seq和H3K27ac ChIP–seq数据的相似菌株或特异菌株。该表表示在IDR峰的200 bp范围内,每个倍数变化临界值内含有局部遗传变异的增强子的百分比。 f,与菌株特异性活性增强子相关的基序,定义为菌株特异性增加的 H3K27ac 位点。 g, 菌株差异可及性和活性增强子的MAGGIE基序突变分析。
编辑推荐:本研究针对烟草诱导肺癌中非编码RNA调控机制不明的关键问题,通过整合基因表达谱分析、表观遗传学技术和体内外功能实验,首次揭示lncRNA ZFAS1通过招募DNMT3A/3B和PRC2复合物沉默相邻抑癌基因ZNFX1的致癌通路,发现SP1/NFkB-ZFAS1-ZNFX1调控轴可作为肺癌治疗新靶点,为吸烟相关肺癌的表观遗传治疗提供理论依据。
Large compendia of gene expression data have proven valuable for the discovery of novel biological relationships. Historically, most available RNA assays were run on microarray, while RNA-seq is now the platform of choice for many new experiments. The da