图20. RNA polII介导的转录过程 只要涉及蛋白质与DNA的互作,lncRNA就有机可乘,因此在转录过程的调控中,lncRNA可以:(1) 通过结合增强子控制TFs与增强子的互作从而调控增强子活性;(2) 通过转录事件抑制RNA polII复合体与启动子结合从而实现转录干扰...
found that ERK2 (extracellular signal鈥搑egulated kinase 2) phosphorylates Ser of RNAPII on poised genes. In murine ESCs exposed to an inhibitor of MEKs, kinases that activate ERKs or those with a genetic deficiency for ERK1 and ERK2, the promoters of ...
The accumulation of DNA:RNA hybrids at both sides of the DSB resembled the RNA Pol II-mediated de novo bidirectional transcription of dilncRNAs from the DSB17, suggesting that dilncRNAs, rather than pre-existing RNA, such as a mRNA, are generating the observed hybrids (Fig. 1a). To further...
绝大多数miRNA的转录是由RNA polymerase II (Pol II)介导的[15],少数由RNA polymerase III (Pol III)介导(图1.3)[16]。RNA Pol II有着多种多样的互作调节蛋白,这使得miRNA在转录这一步骤可以受到精密调控。RNA聚合酶最先合成的是miRNA的初始转录本(primary transcript),称为primary ...
裂殖酵母异染色质建立的机制有2种假说:(1) siRNA机制,即RNA聚合酶II (RNA polymerase II,RNA PolII)先起始转录产生lncRNA,然后RDRC (RNA指导的RNA聚合酶复合体,RNA-directed RNA polymerase complex)识别并结合正在转录的lncRNA,合成双链RNA (double strand RNA,dsRNA),同时RDRC的Cid12亚基催化dsRNA末端多聚腺苷...
It, RNA pol II, catalyzed the incorporation of ribonucleotides with a base ratio similar to that in total nuclear DNA rather than rDNA. Some of the features of mRNA transcription by RNA pol II have already been mentioned. Later a third enzyme, RNA pol III, was found in eukaryote...
Treatment with the RNAP II inhibitor DRB (5, 6-dichloro-1-β-D ribofuranosylbenzimidazole, 100 μM final concentration) was performed in DMEM:F12 (1:1) medium for 6 h. For heat-shock-induced DNA-ChIP experiments, cells were exposed to 42 °C for 15 min, then either mock-...
Pol II: RNA polymerase II ICS: intronic complementary sequence RBP: RNA binding protein m6A: N6-methyladenosine METTL: Methyltransferase-like YTHDC: YTH domain-containing YTHDF: YTH domain family EV: extracellular vesicle HCC: hepatocellular carcinoma ...
RNA 聚合酶 III抑制剂, 577784-91-9价格:￥2450/盒产品详情:描述:ML-60218 is an inhibitor of broad-spectrum RNA pol III with IC50s of 32 and 27 μM for Saccharomyces cerevisiae... 纯度:98% 包装规格:10mg 用途:- 货号:abs823438 品牌:爱必信(absin) ...
To study the impact of CRL4 neddylation, we immunoprecipitated Pol II-bound TCR complexes from cells treated with neddylation inhibitor. This treatment resulted in a complete loss of Pol II ubiquitylation after UV (Fig. 5b), as reported previously11,17. Although recruitment of CSB, CSA and ...