记得在我去新加坡参加 Nanopore Community Meeting 2023 回来后(2023年9月31日),PacBio发布了其全套KINNEX产品线,Kinnex single-cell RNA,Kinnex full-length RNA和Kinnex 16S rRNA。 从2023年10月31日,PacBio宣布Kinnex full-length RNA Kit正式接受预定,到2023年3月24-25日左右,国内测序厂商宣布第一批测试数据下...
Quantoom Biosciences is a full-stack RNA partner for mRNA- and saRNA-based vaccines and therapeutics. Its N-Force toolbox relies on 3 core elements: RNA design, production and delivery.
Labeling efficiency:Approximately 10μg of full-length digoxigenin-labeled RNA is transcribed from 1μg linear template DNA. Assay Time:135 minutes Sample Materials Linearized plasmid DNA: The DNA to be transcribed is cloned into the polylinker site of an appropriate transcription vector which contains...
Full size image As both CPL-1 and viral components are localized at the ER (Fig. 1f and Extended Data Fig. 2f–h), we examined the effects of ER homeostasis on Orsay virus infection in C. elegans. Consistent with the known anti-viral function of exo-RNAi17,18, rde-1 and drh-1 mut...
Full size image Next, we used the technique of MS2 tagging followed by mass spectrometry40to explore the proteins that bind toLETN(schematic description of the assay provided in Supplementary information, Fig. S10a). The results showed that NPM1 was the most enriched binding partner ofLETN(Fig...
Full size image Monocle3 and RNA velocity analysis of cell differentiation relationships In order to clarify the differentiation relationships between TMJC cells, 15 TMJC cell clusters were calculated and divided into 8 differentiation trajectories based on Monocle3 analysis. Clear differentiation pathways...
[69]. APOBEC1 was first identified through the discovery of a C-to-U modification in apolipoprotein B (ApoB) mRNA, which results in the expression of two distinct forms of the protein (a truncated form and a full-length form) [71,72]. The full-length ApoB protein is responsible for ...
Full size image Hydrogels are composed of a water-swollen three-dimensional network that recapitulates the intrinsic properties of the native extracellular matrix (ECM), making them useful for applications in tissue engineering, drug delivery, cellular morphogenesis, among others41,42. The unique physico...
[4,26]. This resulted in the evolutionary preservation of the ancestral forms with the full-length M and S segments. The main driving force behind LBV diversification withinL.pyrrhocorisappears to be a change in TCS, as clades 2, 3, and 4 deviate from the ancestral ACACAAAG form. The ...
Guanine-rich sequences are able to form complex RNA structures termed RNA G-quadruplexes in vitro. Because of their high stability, RNA G-quadruplexes are proposed to exist in vivo and are suggested to be associated with important biological relevance. However, there is a lack of direct eviden...