Bio生物世界 昨天 撰文丨王聪 编辑丨王多鱼 排版丨水成文 环状RNA(circRNA,也叫做环形 RNA),通常由外显子反向剪接而来,在细胞中广泛存在。近期的研究表明,circRNA 的生成受顺式元件和反式因子的调控,并且还与线性 RNA 的形成存在竞争。尽管总体表达水平较低,但其中一部分相对高丰度的 circRNA 能够以独特的作用模式...
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Regulation of an RNA granule during spermatogenesis: acetylation of MVH in the chromatoid body of germ cells. J. Cell Sci. 124, 4346–4355 (2011). CAS PubMed Google Scholar Gustafson, E. A. & Wessel, G. M. Vasa genes: emerging roles in the germ line and in multipotent cells. Bio...
ADARs deaminate adenosine in dsRNA or the A-form helix (DNA: RNA hybrid) [46,47,48]. The introduction of non-canonical base pairs into the dsRNA structure increases complexity, which actually affects the efficiency of adenosine editing (Fig.2). The two bases next to the edited adenosine in...
RNA-seq data quality—A high percentage of reads map to the genome; high numbers of genes identified; full-length gene analysis with minimal 5' or 3' bias Ultra-low input—Start with as little as 1 cell or 10 pg of total RNA (input range: 1–1,000 cells or 10 pg–10 ng of tota...
Full size image Next, we tested the capability of HCR RNA-FISH to detect 3 transcripts simultaneously in the same inflorescence. Using wholemount FISH, APETALA 3 (AP3), AGAMOUS (AG), and SHOOT MERISTEMLESS (STM) were simultaneously probed and detected with previously reported spatial expression...
Guanine-rich sequences are able to form complex RNA structures termed RNA G-quadruplexes in vitro. Because of their high stability, RNA G-quadruplexes are proposed to exist in vivo and are suggested to be associated with important biological relevance. However, there is a lack of direct eviden...
[32]. For example, researchers are exploring the use of TIL therapy alongside immune checkpoint inhibitors to further enhance the anti-tumor immune response. Immune checkpoint inhibitors can alleviate the brakes on the immune system, allowing TILs to exert their full potential in targeting cancer ...
Current single-cell RNA sequencing (scRNA-seq) methods with high cellular throughputs sacrifice full-transcript coverage and often sensitivity. Here we describe Smart-seq3xpress, which miniaturizes and streamlines the Smart-seq3 protocol to substantially
Life of psi: how full-length HIV-1 RNAs become packaged genomes in the viral particles. Virology 454–455, 362–370 (2014). PubMed PubMed Central Google Scholar Pickett, G. G. & Peabody, D. S. Encapsidation of heterologous RNAs by bacteriophage MS2 coat protein. Nucleic Acids Res. 21...