1988 . Extraction of RNA from plants. In: Walker JM, ed. Methods in molecular biology , Vol. 4. New nucleic acid techniques . USA: Humana Press, 437 –446.SLATER, A.: Extraction of RNA from plants. In: WALKER, J. M. (ed.): New Nucleic Acid Techniques, pp. 437-446. The ...
RNA extraction from tissues or cells begins with a process of lysing and denaturing cells to free up total nucleic acids. Whether you’re starting with cultured cells or tissue samples, or working with plants, bacteria, or mammalian cells, Thermo Fisher Scientific RNA...
The method was also effective in extracting RNA from seeds of other cereals including field-grown sorghum and corn. The modified SDS-LiCl method is a robust and highly reproducible RNA extraction method for plant tissues rich in starch and other secondary metabolites. The modified SDS-LiCl method...
Monarch® 总RNA 小量提取试剂盒 Effective May 2, 2024, the Monarch gDNA Removal Columns are now tinted orange to help distinguish from other columns.仅需一款试剂盒,即可实现从多种样本类型中快速、轻松地纯化多达 100 µg 的高质量总 RNA!适用于血液、细胞和组织、唾液以及口腔/鼻咽拭子也...
Extraction of plant RNA This protocol details an RNA preparation for medium-scale, high-purity RNA production from higher plants. It uses hot acid phenol with standard sodium acet... Michael G Salter,Helen E Conlon - 《Methods in Molecular Biology》...
plants derived from the transcriptomes of the 1000 Plants (1KP). The corresponding plant habitats were sourced from the Global Biodiversity Information Facility (GBIF). The bioclimatic variables of these plant habitats were derived from the WorldClim database, i.e., BIO1: annual mean temperature ...
The final RG fraction resulting from the protocol described above can be subjected to regular RNA extraction and subsequently tested for either targeted RNA analyses using RT-qPCR or transcriptome-wide profiling with RNA-seq. 在Bio-protocol期刊查看原文,可以获取更多内容,包括摘要、背景、耗材试剂、仪器软...
The initial organic extraction of the mirVana miRNA Isolation Kit provides a robust front-end purification that removes cell debris and most DNA. Although enrichment of the small RNA fraction can increase sensitivity in many applications, total RNA is usually...
Extraction of the attention contrast matrix To facilitate better model interpretation, we created two additional models. One is the true model, denoted as PlantRNA-FM(+), trained using real TE labels, whereas the other one is the background model, PlantRNA-FM(–), altered using the same dat...
RNAlaterRNA Stabilization Solution is compatible with one-step RNA isolation methods, such as TRIzol Reagent; with glass binding methods, such as Qiagen's RNeasy or the Ambion RNAqueous kit; with acid phenol extraction methods, and with methods that use oligo(dT) selection of mRNA, such as th...