Extraction of total RNA from the leaves of Platycladus orientalis rich in palysaccharides and polyphenol[J]. Journal of Jilin Agricultural University, 2012, 34( 1 ): 76- 80, 89. (in Chinese).WANG S H, XU Q, XU X, et al. Extraction of total RNA from the leaves of Platycladus ...
8. A Novel Method of Total RNA Extraction from Leaves of.China Biotechnology,2013,33(5):107-111 9. The Response to High Magneto-Gravitational Environment of Different Differentiating-Stage Osteoblasts. Chinese Journal of Cell Biology 2013, 35(7): 10. Disrupted calcium homeostasis is involved in ...
There are many protocols used for RNA extraction from plants, which vary according to the composition of the tissue, the part of the plant, the cultivation area and theatmosphere. Here, in Malaysia, we will focus on RNA extraction from the sugarcane leaves (Saccharum officinarum) by using two...
These fungi were propagated in oatmeal broth (2 g/L fine oatmeal powder without agar) for 10 weeks, and dsRNAs were extracted from fungi using a dsRNA extraction kit (ISOVIRUS, NIPPON GENE CO., LTD, Tokyo) and the manufacturer’s instruction. Alternatively, we extracted total RNA and then...
The quality of total RNA isolated from roots,stems,leaves and calli of Populus euphratica Oliv is the foundation of gene expression related researches using the RNA.In this paper,seedling roots of two-year-old P.euphratica were used as test materials,and CTAB-PVP extracting buffer was developed...
Absrtact ：Taking tomato variety Moneymaker （MM ）as the material ，compared the extraction effects of three methods including kit method ，Trizol method and Trizol improved method on total RNA from tomato leaves and fruits at different stages ，and measured the concentration ，purity and integrity ...
method.In above four methods,only modified CTAB-LiCl method could extract the higher quality total RNA of leaves of tung tree.For these reasons,modified TRIzol method and modified CTAB-LiCl method could be regarded as preferred method for the RNA isolated respectively from leaves and kernels of ...
(cDNA-AFLP),and RNA blotting.The A_260/A_230 ratios were higher than 2.0,and the A_260/A_280 ratios ranged from 1.65 to 1.92.In addition,the protocol is safe,convenient and proved to be widely applicable,since it was successfully employed for RNA extraction from citrus leaves,immature ...
The mature leaves of apple trees are rich in polyhexose,polyphenols and other secondary metabolism compounds which prevent RNA extraction.The genomic RNA samples of mature leaves and phloem of field-grown apple in different seasons were isolated by the improved method.Total RNA of high purity,higher...
The modified SDS-LiCl method is a robust and highly reproducible RNA extraction method for plant tissues rich in starch and other secondary metabolites. The modified SDS-LiCl method successfully extracted high yield and quality RNA from mature, developing, and germinated seeds, leaves, and roots ...