RNAlater-treatment for stabilization of mRNA profiles in captured cells RNA extraction from captured leukocytes by a phenol-free method in as little as 45 minutes TURBO DNase™ included for highly pure RNA (optional step) Ambion’s new LeukoLOCK™ Total RNA ...
The same advantages that magnetic beads provide for viral RNA isolation also apply to high throughput total RNA isolation from cultured cells. No organic extraction or precipitation steps are required, there are no filters to clog, RNA is eluted in a very small...
For RNA extraction from mammalian cells, the growth medium was removed and cells were washed once with PBS. Subsequently, cells were scratched from the culture dish, collected in PBS and pelleted by centrifugation (5 min, 500 RCF, RT). The supernatant was removed, the pellet resuspended in...
We demonstrate on-chip, differential DNA and RNA extraction from a single cell using a microfluidic chip and a two-stage lysis protocol. This method enables direct use of the whole extract, without additional washing steps, reducing sample loss. Using th
[16]Kasimanickam VR, Kasimanickam RK. An efficient approach for RNA extraction from boar sperm and seminal plasma[J]. Bio Protoc, 2019, 9(13): e3284. DOI: 10.21769/BioProtoc.3284. [17]Zhu C, Chen F, Zhang S...
总RNA的提取(英文课件)TotalRNAextractionfromplant 江燕 AnRNase-freeenvironmentiscritical:WorkinanRNaseFREEareaandworkoniceunlessotherwiseindicated.Alwayswearcleangloves(don´ttouchyourface/hairor"dirty"surfaceswithyourglovesandassumetheyremainnuclease-free).UsefiltertipsandotherRNase-freeconsumables.Makesurethe...
英文名称:Total RNA Extraction Kit from Cultured Cells/Bacteria 产品货号:WH0060 产品规格:50次 本试剂盒采用高效、专一结合核酸的离心吸附柱和独特的缓冲系统,可从各种类型的培养细胞中快速提取总RNA,可同时处理大量不同样品。30-40分钟内即可完成反应,提取的总RNA纯度极高,没有蛋白质和其他污染。
The successful separation of crypts from villi was confirmed by qPCR for Apoa4 (villi) and Defa5 (crypts) using cDNA prepared from Venus negative cells. 2.5. RNA extraction and quantitative RT-PCR RNA extraction from FACS-sorted cells was performed using an RNeasy Micro Plus Kit (Qiagen). ...
Our method uses an RNase inhibitor and combines different protocols using guanidium thiocyanate–phenol extraction. It enables reproducible isolation of RNA with an RNA integrity number around 9. 展开 关键词: RNA Pancreas Mouse RNA purification RNA integrity number ...
SEPARATION OF RNA AND DNA FROM CELL 优质文献 相似文献In-situ sampling and separation of RNA from individual mammalian cells. In this investigation RNA was directly sampled and separated at the single-cell level (without extraction) by capillary electrophoresis (CE). Laser-induced... F Han,SJ Li...