RNA base editing enables a precise, reversible and doseable change of genetic information. Here, authors propose base editing as a method to control post-translational modifications via removal of regulatory phosphorylation and acetylation sites, applying the approach to modulation of the JAK/STAT pathw...
Direct identification of A-to-I editing sites with nanopore native RNA sequencing 今天给大家带来一篇新加坡南洋理工大学有关RNA修饰使用纳米孔测序进行检测的文章。作者使用了牛津纳米孔直接对RNA测序,用于鉴定天然转录组中含肌苷的位点,提出了Dinopore (Detection of inosine with nanopore sequencing)。作者训练了卷...
Enzymatic conversion of adenosine to inosine is an RNA editing mechanism for post-transcriptional diversification of mRNA. A new chemical tagging–reverse transcription method leads to the identification of new A-to-I RNA editing sites in the human genom
1. Zhang P, Zhu Y, Guo Q, et al. On the origin and evolution of RNA editing in metazoans[J]. Cell Reports, 2023, 42(2). 2. Gray, M.W. (2012). Evolutionary origin of RNA editing. Biochemistry 51, 5235-5242. 10.1021/bi300419r. 3. Liscovitch-Brauer, N., Alon, S., Porath,...
2023年2月14日,华大生命科学研究院李启业与浙江大学张国捷联合其他国内外合作者在Cell Reports杂志在线发表题为On the origin and evolution of RNA editing in metazoans的研究论文,揭示了ADAR介导的RNA编辑机制是后生动物祖先的重要演化创...
and reduced RNA editing due to decreased expression of these enzymes [27,114]. In some cancers, there is no clear correlation between the level of RNA editing and the expression of RNA editing enzymes [25]. The extent of RNA editing and the significance of RNA editing sites vary among diff...
Eight RNA editing site’s filters to filter out the most possible spurious sites. Quality Control Filter (contain quality filter and coverage filter); Editing Type Filter Splice Junction Filter; Known SNP Filter; Repeated Regions Filter; DNA-RNA Filter; ...
(2016). RES-Scanner: a software package for genome-wide identification of RNA-editing sites. Gigascience 5, 37. 10.1186/s13742-016-0143-4. 9. Liddicoat, B.J., Piskol, R., Chalk, A.M., Ramaswami, G., Higuchi, M., Hartner, J.C., Li, J.B., Seeburg, P.H., and Walkley, ...
RNA-editing is an important post-transcriptional RNA sequence modification performed by two catalytic enzymes, "ADAR"(A-to-I) and "APOBEC"(C-to-U). By utilizing high-throughput sequencing technologies, the biological function of RNA-editing has been acti
2. Identification of editing sites from RNA-seq datavariant calling:用GATK中的UnifiedGenotyper来call variants,与普通的variant calling不同,这里采用了比较宽松的选项:stand_call_conf 0, stand_emit_conf 0, and output mode EMIT_VARIANTS_ONLYremove all known SNPs:利用dbSNP数据,过滤已知的SNPs...