RNA interference (RNAi) is a powerful technique for gene silencing in Drosophila. RNAi involves the introduction of double-stranded RNA (dsRNA) that is complementary to the mRNA of the target gene. The dsRNA is processed by the RNA-induced silencing complex (RISC), which degrades the target ...
Further aspects that complicate the conceptual framework of gene essentialty include the observation that deletion of non-essential genes can be beneficial to the organism under particular experimental conditions, in comparison to others (Qian et al., 2012). Furthermore, the deletion of some genes,...
Modern genome editing on the other hand is a faster and more targeted way to alter DNA, making it safer to modify DNA and provide predictable phenotypes (Martineau, 2001). Up until the 1970 s, SB was the only genetic modification method available that was nonetheless essential in sustaini...
It could be very beneficial for other experimental organisms as well that are sensitive to environmental factors or chemicals. We used piggyBac as the vector since it can integrate into the genomes of a wide range of insects [17] and organisms [23]. In Drosophila, the piggyBac vector often...
The use of chemical pesticides for insect pest control, often applied without legal supervision, has led to environmental issues such as the death of beneficial insects, parasitoids, and predators, and pesticide residues in the soil [10]. Studies aimed at discovering active compounds from natural ...
In particular, the zebrafish (Danio rerio) has been established as one of the most important model organisms for cancer research. This model is particularly suitable for live cell imaging and high-throughput drug screening in a large-scale fashion. Thanks to the recent advances in genome editing...