RNA editingbacterianon-genetic variationhokBtoxin-antitoxinpersistenceantibiotic tolerancetadAADATADARDNA harbors the blueprint for life. However, the instructions stored in the DNA could be altered at the RNA level before they are executed. One of these processes is RNA editing, which was shown to...
2. Bar-Yaacov D, Mordret E, Towers R, Biniashvili T, Soyris C, Schwartz S, Dahan O, Pilpel Y:RNA editing in bacteria recodes multiple proteins and regulates an evolutionarily conserved toxin-antitoxin system.Genome Res2017, 27:1696-1703. 3. Liu H, Wang Q, He Y, Chen L, Hao C, ...
2020年8月21日,上海交通大学农业与生物学院朱勃/陈功友合作团队在国际著名学术期刊PLoS Pathogens发表了题为A-to-I RNA editing in bacteria increases pathogenicity and tolerance against oxidative stress的研究论文,揭示了原核细菌通过 A-to-I 的RNA编辑适应环境变化的新机制。 该研究团队通过自行开发的Python脚本对...
et al. RNA editing in bacteria recodes multiple proteins and regulates an evolutionarily conserved toxin-antitoxin system. Genome Res. 27, 1696–1703 (2017). Article PubMed PubMed Central CAS Google Scholar McMahon, A. C. et al. TRIBE: hijacking an RNA-editing enzyme to identify cell-...
To generate a detectable edit, we used a Cas9 cytosine base editor that uses rAPOBEC1 to convert any C into a T within an editing window in the target26 (Fig. 1c and Extended Data Fig. 1a). The Cas9 comes from the CRISPR1 locus of Streptococcus thermophilus (Sth1Cas9), where this ...
in various cellular processes. There are two main types of RNA editing events: adenosine to inosine (A-to-I) editing, catalyzed by ADARs on double-stranded RNA or ADATs on tRNA, and cytosine to uridine (C-to-U) editing catalyzed by APOBECs. This article provides an overview of the ...
综上所述，该研究报道了RADAR系统复杂且新颖的超分子复合体组装细节，发现了RADAR实现底物装载、运输及修饰的多酶偶联机制，并对开发基于RADAR的base-editing工具、纳米颗粒载体和新型抗菌疗法等提供了基础。中科院生物物理所高艺娜副研究员为本文第一作者，高璞研究员为本文通讯作者。原文链接：https://doi.org/10....
2019年1月30日,华中农业大学张安定,金梅林,韩丽及同济大学项耀祖共同通讯在Nucleic Acids Research在线发表题为“The prokaryotic Argonaute proteins enhance homology sequence-directed recombination in bacteria”的研究论文,该研究发现Nagonobacterium gregoryi Argonaute(NgAgo)以80-100%的效率增强巴斯德氏菌和大肠杆菌中...
Mutation frequencies of cells transformed with the vector itself or with a tac-AID expression vector were determined in parallel. Strikingly, APOBEC1-transformed bacteria grown in the presence of the transcriptional inducer IPTG displayed massively elevated frequencies of RifR mutation (Figure 1A). ...
in thesubunit compositionof RNA polymerases from different sources, the basic structure and organization of the RNA-making machinery are conserved. For example, in bacteria, the RNA polymerase consists of four core subunits and asigma factor, whose role is to specify transcript start points, ...