2020年8月21日,上海交通大学农业与生物学院朱勃/陈功友合作团队在国际著名学术期刊PLoS Pathogens发表了题为A-to-I RNA editing in bacteria increases pathogenicity and tolerance against oxidative stress的研究论文,揭示了原核细菌通过 A-to-I 的RNA编辑适应环境变化的新机制。 该研究团队通过自行开发的Python脚本对...
2. Bar-Yaacov D, Mordret E, Towers R, Biniashvili T, Soyris C, Schwartz S, Dahan O, Pilpel Y:RNA editing in bacteria recodes multiple proteins and regulates an evolutionarily conserved toxin-antitoxin system.Genome Res2017, 27:1696-1703. 3. Liu H, Wang Q, He Y, Chen L, Hao C, ...
In addition, the editing events T408A on the enterobactin iron receptor protein XfeA act as switches by controlling the uptake of Fe3 in response to the concentration of iron in the environment. Even though bacteria have fewer editing events, the great majority of ...
RNA-editing Cas13 enzymes have taken the CRISPR world by storm. Like RNA interference, these enzymes canknock down RNA without altering the genome, but Cas13s have higher on-target specificity. New work fromKonermann et al.andYan et al.describes new Cas13d enzymes that average only 2.8 kb...
To generate a detectable edit, we used a Cas9 cytosine base editor that uses rAPOBEC1 to convert any C into a T within an editing window in the target26 (Fig. 1c and Extended Data Fig. 1a). The Cas9 comes from the CRISPR1 locus of Streptococcus thermophilus (Sth1Cas9), where this ...
RNA-binding proteins (RBPs) are central to most if not all cellular processes, dictating the fate of virtually all RNA molecules in the cell. Starting with pioneering work on ribosomal proteins, studies of bacterial RBPs have paved the way for molecular
They show that many editing level differences between the speci... Knud,Larsen,Kaja,... - 《Biochemical & Biophysical Research Communications》 被引量: 3发表: 2016年 The occurrence, characteristics, and adaptation of A-to-I RNA editing in bacteria: A review A-to-I RNA editing is a very ...
in various cellular processes. There are two main types of RNA editing events: adenosine to inosine (A-to-I) editing, catalyzed by ADARs on double-stranded RNA or ADATs on tRNA, and cytosine to uridine (C-to-U) editing catalyzed by APOBECs. This article provides an overview of the ...
了解更多: RNA-guided gene editing Nature Methods, 27 February 2013 | doi:10.1038/nmeth.2389 Targeted gene modification can be guided by programmable RNA in bacteria, zebrafish and mammalian cells.
综上所述，该研究报道了RADAR系统复杂且新颖的超分子复合体组装细节，发现了RADAR实现底物装载、运输及修饰的多酶偶联机制，并对开发基于RADAR的base-editing工具、纳米颗粒载体和新型抗菌疗法等提供了基础。中科院生物物理所高艺娜副研究员为本文第一作者，高璞研究员为本文通讯作者。原文链接：https://doi.org/10....