RNA interference (RNAi) is a powerful technique for gene silencing in Drosophila. RNAi involves the introduction of double-stranded RNA (dsRNA) that is complementary to the mRNA of the target gene. The dsRNA is processed by the RNA-induced silencing complex (RISC), which degrades the target ...
Cells are coloured according to the assigned cell type using the lowest cut off (that is, taking into account cells with at least 100 transcripts). We do not lose any cell type cluster when applying higher transcript cut offs, nor do we generate new clusters of low transcript cells when ...
Our results show that the ZBD is both essential for ProRS catalytic activity and contributes to stability. Its presence in all four major archaeal sub-clusters and certain bacterial phyla is potentially instructive with respect to environmental or other forces driving its origin. Hyperthermic conditions...
encoding components of the PI3K complex and the vacuolar protein sorting complex, are required for viability ofS. macrospora(Voigt et al.2014). These results suggest that inS. macrospora, autophagy is an essential and constitutively active process required to maintain high energy levels ...
E. CRISPR/Cas9 gene-editing approach for the discovery of essential genes in cell culture. F. Knockdown by RNA-interference for the discovery of essential genes in C. elegans. Chemical mutagenesis studies of C. elegans (Brenner, 1974; Clark et al., 1988; Johnsen and Baillie, 1991) revealed...
Scarring ts after in different clones (for example, the yellow scar is present in one clone injecting the yolk or cell of the zygote wi as9 RNA or protein, and from fish R1 and four clones from fish R2), the chance of introducing a single-guide RNA (sgRNA) that targets GFP (Fig. ...
Pneumocystis jirovecii is the species that infects immune-compromised humans, whereas Pneumocystis carinii (Pc)2 represents the parallel species utilized in the widely studied rodent models. Studies of Pc have proven extremely useful, as all of the therapies for the treatment of human disease have ...
Modern genome editing on the other hand is a faster and more targeted way to alter DNA, making it safer to modify DNA and provide predictable phenotypes (Martineau, 2001). Up until the 1970 s, SB was the only genetic modification method available that was nonetheless essential in sustaini...
Spirulina is also a prospective component of both terrestrial and space-based life support systems. Here, we review current breakthroughs in spirulina research and clarify fallacies that can be found in both professional literature and public media. Keywords: Arthrospira; Limnospira; Spirulina; ...
This motif is identical to the orthologous C. elegans cyp-13A11 and cyp-13A12 heme-binding sites (Table 2). However, functionally or structurally validated data such as crystal structures are currently unavailable for the C. elegans orthologous Cyps. Keller et al. (2014) revealed that Cel-...