根据Abcam官网提供的RNA dot bloting protocol,进行实验在实验过程中适当更改实验条件,形成实验笔记。 RNA dot blot 一、试剂 RNA样本 无RNase水 TBST(1x TBS,0.1% Tween-20) 封闭液一抗 二抗(山羊抗兔 IgG-HRP, ab97051, 或山羊抗小鼠 IgG-HRP) ECL显影液 链霉亲和素-AlexaFluor488 亚甲蓝染色缓冲液(0.4...
RNA dot blot is a technique similar to northern blotting; however, RNA samples are not separated by electrophoresis. Instead, they are spotted onto a membrane. RNA sample RNase-free water TBST (1x TBS, 0.1% Tween-20) Blocking Solution Primary antibody Secondary antibody (goat anti-rabbit IgG-...
11. 清洗膜并采集图像。参考资料：RNA Dot Blot Protocol | Abcam
16,用ddH2O清洗至背景大致干净即可,约30-60s 17,白光成像采集亚甲蓝染色后的图像作为负载对照。
RNA Dot blot 实验流程 1.将已知浓度的样本样品，用无酶离心管进行梯度稀释（并上操作）2.短暂离心，然后将RNA样品95℃加热3分钟，结束后迅速转到冰上冷却，防止重新形成二级结构。3.用1ml枪头粗端在NC膜上留下圆形印痕并用铅笔描圈，以引导加样，并将 NC膜裁剪成合适的尺寸，转移至干净的培养皿中。4.取2...
下面这套 protocol 你先收藏,应用范围和浓度问题,师兄再和你细细说。 师兄 一招提效! 同时提 RNA DNA 蛋白质 TRIzol 试剂适用于从细胞和组织中快速分离 RNA。最大特点是可同时分离一个样品的 RNA、DNA、蛋白质。 提取的 RNA 无蛋白质和 DNA 污染,可用于 Northern Blot,dot blot,ployA 筛选,体外翻译,RNase ...
摘要: Posttranscriptional modification of mRNAs plays an important role in establishing the functional diversity of the proteome. The m6A modification is found in many species of RNA, including tRNA, mRNA,...关键词: Dot blot Immunoprecipitation Posttranscriptional modifications m6A ...
Protocol The Nucleic Acid Protocols Handbook pp 71-72 Preparation of RNA Dot Blots Rachel聽HodgeAffiliated withDepartment of Botany, University of Leicester * Final gross prices may vary according to local VAT. Get Access Abstract RNA dot hybridizations were first described by Kafatos et al. (1...
Dot blotImmunoprecipitationPosttranscriptional modificationsm6APosttranscriptional modification of mRNAs plays an important role in establishing the functional diversity of the proteome. The m6A modification is found in many species of RNA, including tRNA, mRNA,......