RNA-seqleaf sheath cellsresistantricesingle-cellThe brown planthopper (BPH) (Nilaparvata lugens) sucks rice sap causing leaves to turn yellow and wither, often leading to reduced or zero yields. Rice co-evolved to resist damage by BPH. However, the molecular mechanisms, ...
c Enrichment of accessibility of proximal regulatory elements for marker genes as identified by single-cell RNA-seq. Dot diameter, proportion of cluster cells with proximal regulatory elements for a given gene; colour, mean accessibility across cells in that cluster. d UMAP visualization of cell ...
The single-cell transcriptome atlas enabled us to identify most of the major cell types of the rice radicle (Supplementary Note 2). Clusters 1, 4 and 9 belonged to the epidermis/root hair population (Fig. 1a, see below). These clusters were separated from other cell clusters on the UMAP ...
Figure 1. Single-cell RNA-seq and cluster annotation of rice root tips. (A) Overview of rice scRNA-seq and cluster annotation workflow. Protoplasts were isolated from 5-mm root tips of rice Nip and 93-11, respectively. scRNA-seq libraries were generated using the 10x Genomics platform follow...
Here, we have used high-throughput single-cell RNA sequencing to profile 37571 rice inflorescence cells and constructed a genome-scale gene expression resource covering the inflorescence-to-floret transition during early reproductive development. The differentiation trajectories of florets and AMs were ...
Since translation is a most energy‐demanding process, fine-tuning the regulation of this process is necessary not only for the faster production of the desired proteins but also to adjust the level of protein synthesis according to the actual demand. Stage and cell‐specific global changes in...
Rhoades KL, Singh N, Simon I, Glidden B, Cedar H, Chess A: Allele-specific expression patterns of interleukin-2 and Pax-5 revealed by a sensitive single-cell RT-PCR analysis. Curr Biol. 2000, 10 (13): 789-792. Article PubMed CAS Google Scholar Wittkopp PJ, Haerum BK, Clark AG:...
Single cell-type tranome profiling reveals genes that promote nitrogen fixation in the infected and uninfected cells of legume nodules 来源: PLANT BIOTECHNOLOGY JOURNAL DOI 2020影响因子: 9.803 第一作者: 1.王龙龙,2.周宇 通讯作者: 端木德强
(902 nts, p-value = 1.16 × 10− 24, two-tailed Student’st-test). This longer average length of novel lncRNAs is likely due to the fact that we removed the low-confidence single exon transcripts during the prediction process. Among the different categories of lncRNAs, ...
Then, we performed quantitative real-time RT-PCR (qRT-PCR) analysis to determine whether these 25 genes exhibited the expected changes in expression under the same ER stress conditions used for RNA-Seq analysis (5 μg/mL TM for 2 hr). As shown in Figure3, the expression patterns of 17 ...