In vitro transcribed tRNA(Phe) analogues from Escherichia coli containing up to four randomly distributed A, G, U or C phosphorothioated nucleotides were used to investigate contact patterns with the ribosome in the A and P sites. The tRNAs were biologically active. Molecular iodine (I2) can ...
This view had its origin in part from the expectation that the proteins, by analogy with soluble enzymes, would play the crucial role in recognition of tRNA as well as in other ribosomal functions. It also was based on the fact that the early affinity labeling experiments identified primarily ...
Functional design of ribosomes with mutant ribosomal RNA (rRNA) can expand opportunities for understanding molecular translation, building cells from the bottom-up, and engineering ribosomes with altered capabilities. However, such efforts are hampered by cell viability constraints, an enormous combinatori...
The interaction of EF-G with ribosomal elements implicated in stimulating catalysis, such as the L10-L12 stalk and the L11 region, and of domain IV of EF-G with the tRNA at the peptidyl-tRNA binding site (P site) and with mRNA shed light on the role of these elements in EF-G ...
Dom34:Hbs1 promotes subunit dissociation and peptidyl-tRNA drop-off to initiate no-go decay. Science 330, 369–372 (2010).This study together with ref. 37 provides rigorous biochemical evidence that the Dom34–Hbs1 complex mediates the splitting of translationally stalled ribosomes. Article CAS...
IF-1promotes dissociation of the ribosomal subunits. IF-3 then binds to the free 30S subunit and prevents its reassociation with the free 50S subunit. 2. Formation of the 30S Initiation Complex (1) Binding of IF-1 and IF-2, alongside IF-3. (2) Binding of mRNA and fMet-tRNAfMet to...
Recently, several complete ribosomal structures have been determined, of the E. Coli ribosome (Schuwirth et al., 2005), and from T. thermophilus with tRNA (Korostelev et al., 2006) and with tRNA and mRNA (Selmer et al., 2006). These structures are some of the largest determined by X...
In addition, GTP was necessary with 80-S ribosomes and 40-S plus 60-S particles but not with 40-S subunits. Similar incubations with N-acetylphenylalanyl-tRNA led to significant binding of the N-acylaminoacyl-tRNA to 40-S subunits and to a lesser extent to 80-S ribosomes or to 40-...
Initiation of Protein Synthesis from the A Site of the Ribosome Positioning of the translation initiation complex on mRNAs requires interaction between the anticodon of initiator Met-tRNA, associated with eIF2-GTP and 4... JE Wilson,TV Pestova,CUT Hellen,... - 《Cell》 被引量: 644发表: 2000...
a toolset for mining multi-dimensional features of the translatome with ribosome profiling data - bonomali/RiboMiner