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The site within an actively translating ribosome that is usually associated with the tRNA attached to the growing peptide chain. Codon periodicity The three-nucleotide pattern of ribosome occupancy, reflecting mRNA translocation in the ribosome by codon as translation occurs. ...
Functional design of ribosomes with mutant ribosomal RNA (rRNA) can expand opportunities for understanding molecular translation, building cells from the bottom-up, and engineering ribosomes with altered capabilities. However, such efforts are hampered by cell viability constraints, an enormous combinatori...
is consistent with the facts about interactions of the protein L7/L12 with the elongation factors (EF-Tu and EF-G) involved in tRNA binding and translocation, as well as with the data available on the participation of proteins, S3, S5, S10, S14 and S19 in the formation of tRNA sites. ...
It is generally believed that eukaryotic ribosomes first associate with mRNA in the cytoplasm. However, we show with chromosomal immunostaining and in situ hybridization that ribosomal subunits are present at transcription sites of Drosophila salivary gland chromosomes. Immunostaining was carried out with ...
This view had its origin in part from the expectation that the proteins, by analogy with soluble enzymes, would play the crucial role in recognition of tRNA as well as in other ribosomal functions. It also was based on the fact that the early affinity labeling experiments identified primarily ...
P.A. Czernilofsky, E. Collatz, A.M. Gressner, I.G. Wool, E. Kuchler, Identifi- cation of the tRNA-binding sites on rat liver ribosomes by affinity labeling, Mol. Gen. Genet. 153 (1977) 231-235.Czernilofsky AP, Collatz E, Gressner AM, Küchler E, Wool IG (1977) Identification...
Effect of edeine on aminoacyl-tRNA binding to ribosomes and its relationship to ribosomal binding sites 1. The antibiotic edeine, an oligopeptide produced by Bacillus brevis, possesses a Mg 2+ concentration-dependent inhibitory activity with respect to messen... W Szer,Z Kurylo-Borowska - 《Bioc...
AUG Cap Codon fMet-tRNA Initiation codon Initiation factors Methionine mRNA P-site Ribosome 被引量: 9 摘要: Provided are ribosome binding sites having an increased number of adenine (A) and thymidine (T) residues as compared to the naturally occurring ribosome binding sites from which they are...
The ribosome has long been considered as a consistent molecular factory, with a rather passive role in the translation process. Recent findings have shifted this obsolete view, revealing a remarkably complex and multifaceted machinery whose role is to or