1a). We used the ANSI convention41 when indexing the Zernike coefficients, customizing aberrations by using different Zernike coefficients for different datasets acquired from different microscopes. For all experimental datasets, we added aberrations up to the 4th Zernike order (i.e., M = 14)...
from the view of an astronomer and in it he describes “the form and brightness of the rings or rays surrounding the image of a star as seen in a good telescope”. Despite writing in a different scientific field, these observations are relevant to other optical systems including micro...
(b) Magnified views from the region enclosed in the white dashed box in (a) under different microscopes. Huygens- represents that the images were deconvoluted by Huygens Professional (Scientific Volume Imaging, The Netherlands). (c) The Fourier transforms of images obtained by the corresponding ...
Single-molecule based super-resolution microscope delivering ten-times the resolution of standard light microscopes.
Single-molecule based super-resolution microscope delivering ten-times the resolution of standard light microscopes.
X-ray CT microscopes can now reach submicron voxel resolutions without the need for a synchrotron to provide thousands of cross-sectional images non-destructively33. Although their use in corrosion degradation studies is increasing, most studies focus on large objects typically embedded in a media tha...
We further demonstrate simultaneous large field-of-view two-photon and three-photon imaging, subcortical imaging in the mouse brain, and whole-brain imaging in adult zebrafish. The demonstrated techniques can be integrated into typical multiphoton microscopes to enlarge field of view for system-level ...
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Super-resolution imaging has, for more than seventy years, gradually evolved to produce advanced methods for enhancing the resolution of images beyond the diffraction limits. Notwithstanding its foreseeable practical capabilities, we noted that this tech
The vast majority of sub-3 Å structures submitted to the PDB were determined with 300 kV cryo-transmission electron microscopes (cryo-TEMs) that were specifically designed for high-resolution data collection at cryogenic temperatures. In fact, it is safe to say that 300 kV has been treated ...