from the view of an astronomer and in it he describes “the form and brightness of the rings or rays surrounding the image of a star as seen in a good telescope”. Despite writing in a different scientific field, these observations are relevant to other optical systems including micr...
On top of this intrinsic domain structure, bits were written thermomagnetically using different laser powers. These samples have been imaged in six different laboratories employing both home-built and commercial magnetic force microscopes. The resolution obtained with these different microscopes, tips and...
Calculated and measured resolution figures are presented for confocal microscopes with different pinhole sizes and for nonlinear (2-and second harmonic) microscopes. A modest degree of super-resolution is predicted for a confocal microscope but in practice this is not achievable and confocal fluorescence...
of rotational parameters in each frame reconstruction. This refinement produced a 0.4-Å improvement in the resolution of the first frame reconstruction. Therefore, only a mild rapid motion effect occurred on the cellular lamellae, which was different from single-particle data. This difference was ...
SmarAct offers several plug-and-play kits for different open-source microscopes (e.g., NanoPro 1.0, MiCube, K2 TIRF a.o.) With the SmarAct's superresolution kits you receive complete and ready-to-use sets for sample manipulation consisting of positioning systems, control units and required...
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or the use of the method for the analysis of image multisets formed by related images collected with the same platform or for image fusion scenarios involving images of different platforms. Besides, MCR scores and loadings are excellent compressed, noise-filtered, and compound-specific image informat...
and reduces the FWHM of individual emitters (Fig.1cvertical continuous lines). A comparison of the shrinkability of MSSR0applied to Gaussian and Bessel PSFs are shown in Supplementary Fig.S9. The reduction of FWHM of Bessel PSF at different wavelengths of the visible spectrum are shown in Sup...
Advancements in microscopes have been more recently extended to enabling the viewing of objects on a nanoscale and objects that are invisible using traditional instruments. An important development in these advancements is the X-ray microscope. The utilization of X-rays, which are electromagnetic waves...
By characterizing the intensity and polarization distributions of the two focused beams, we verify the feasibility of the subtractive imaging method in polarization dependent SHG microscopy. The resolution and contrast enhancement in different biological samples is demonstrated. This work will open a new ...