A non-periodic sample, which also contains a periodic structure with a period smaller than the resolution limit of the optical microscope used for imaging it, was illuminated with the cone of white-light produced by a ring-shaped condenser with a numerical aperture larger than the objective lens...
a resolution measure that overcomes the limitations of Rayleigh’s criterion was proposed. It predicts that the resolution of an optical microscope is not limited and that it can be improved by increasing
This idea was further extended to the superlens and hyperlens, which was applied in nano-lithography and helped to improve the resolution of an optical microscope9,10,11,12,13,14,15,16. Based on fluorescence radiation manipulations, some super-resolution microscopes, including stimulated emission ...
An optical microscope set on a high magnification may produce an image that is blurred and yet it is still at the maximum resolution of the objective lens. The numerical aperture of the objective lens affects the resolution. This number indicates the ability of the lens to gather light and ...
An optical microscope composed of an objective lens (NA = 0.4, ×10, UPLSAPO10X, Olympus Corp.), a tube lens (f = 180 mm, TTL180-A, Thorlabs, Inc.), and an sCMOS camera (6.5 μm, 2048 × 2048, Zyla 4.2 PLUS, Oxford Instruments plc) was used for speckle ...
To provide a microscope device capable of resolving a microscopic pattern beyond optical resolution of the microscope device. A microscope device includes, an image sensor, an enlarging optical system forming a magnified image of at least part of a target of observation on the image sensor, and ...
The numerical aperture (NA) is related to the refractive index (n) of a medium through which light passes as well as the angular aperture (α) of a given objective (NA = n sinα). The resolution of an optical microscope is not solely dependent on the NA of an objective, but ...
Super-resolution microscopy is an optical imaging technology that overcomes the diffraction limit of light and allows the visualization of subcellular structures and dynamics in greater detail than can be achieved with conventional optical microscopy. A resolution of 30 nm is possible usingSTED(stimulated...
A far-field optical microscope capable of reaching nanometer-scale resolution using the in-plane image magnification by surface plasmon polaritons is presented. The microscope utilizes a microscopy technique based on the optical properti... II Smolyaninov,CC Davis - US 被引量: 186发表: 2008年 Com...
Near-field scanning optical microscopy (NSOM) reveals membrane nanoscale organization. (A) Principle of operation of a NSOM setup built on a confocal microscope. In NSOM, an Al-coated hollowed optical fiber is a probe that raster scans laterally a sample while locally exciting fluorophores exclusive...