Relative gene expression by real-time PCR expressed as fold change in HAPE-p and HLs.Zahara AliAastha MishraRahul KumarPerwez AlamPriyanka PandeyRekhbala RamTashi ThinlasGhulam MohammadM. A. Qadar Pasha
Normalization of gene expression data refers to the comparison of expression values using reference standards that are consistent across all conditions of an experiment. In PCR studies, genes designated as "housekeeping genes" have been used as internal reference genes under the assumption that their ...
CLIC quantification of EGFR protein levels, normalized to GAPDH protein levels, was compared to quantification of EGFR RNA expression by qPCR, with GAPDH as a reference gene. We found that cells treated with EGFR siRNA, but not with negative control ntRNA, had a significant decrease in EGFR ...
Reverse transcription quantitative real-time PCR (RT-qPCR) is a well-established method for analysing gene expression. Most RT-qPCR experiments in the field of microbiology aim for the detection of transcriptional changes by relative quantification, which means the comparison of the expression level of...
Normalized gene expression levels (log2-transformed fold change) in pancreatic ductal adenocarcinoma (PDAC) samples relative to normal pancreas (NP) and ch... G Carmen 被引量: 0发表: 2013年 Gene expression levels as predictive markers of outcome in pancreatic cancer after gemcitabine-based ...
Impact of nicotine on processes involved in cell stress, damage, and fate and gene expression changes in young and old HAoSMCs. A The response to various concentrations (log10 scale) of aqueous extracts is plotted as the fold change in intensity (log2 and original scales on left and right ...
MicroRNAs, a class of small, non-coding RNAs, play important roles in plant growth, development and stress response by negatively regulating gene expression. Moringa oleifera Lam. plant has many medical and nutritional uses; however, little attention has
The plasma AUCs0–inf of paclitaxel were similar after complete gene knockout of Cyp3a and inhibition of CYP3A4 by ritonavir, but the plasma AUC0–inf of docetaxel was slightly lower after ritonavir inhibition than upon Cyp3a knockout. The difference is not substantial (Table 1), but it can...
We did not observe salvA-mediated changes in KOR-related gene expression in the VTA, suggesting that KORs to this area are unchanged, whereas dynorphin levels in NAc efferents to the VTA are decreased. The effect of decreased KOR expression on intrinsic NAc neurons is not well understood, in ...
Analyzing change in the frequencies of single-nucleotide polymorph- isms (SNPs) sampled randomly from the genome offers an alternative to protein substitution rates, one that is not limited to gene regions amplifiable with conserved primers and effective for recent divergence times. Here we employ ...