Sabine CharronFrançois RoubertieDavid BenoistVirginie DubesStephen H. GilbertMarion ConstantinDelphine VieillotDelphine ElbesBruno QuessonPierre Bordachar
Reverse transcription quantitative real-time PCR (RT-qPCR) is a well-established method for analysing gene expression. Most RT-qPCR experiments in the field of microbiology aim for the detection of transcriptional changes by relative quantification, which means the comparison of the expression level of...
aIn this study, changes in the expression levels of proteins was normalized using the PLGS auto normalization function.With the control samples as calibrator, the relative quantification analysis approach yielded the average relative fold change of the identified proteins among patient 在这项研究,在蛋白...
SPIOMET treatment was accompanied by a 3.4-fold rise of circulating GDF15 (on average, from 308 to 1045 pg/mL) and by a concomitant lowering of CRP and insulin concentrations towards normal, so that the relative GDF15 levels became markedly abundant. Post-OC, the relatively low GDF15 ...
However, it seems unlikely that inhibitory plasticity plays a major role in expression of the OD shift, which appears to be fully manifest by a change in TC excitatory synaptic transmission, at least after 3 days of MD. Our findings do not contradict abundant evidence for a role for ...
2D). In the Uric acid excretion pathway, the threonine supplementation group had the highest expression, and the normal protein control group had the lowest expression (Fig. 2E). Fig. 2 Effects of amino acid combinations with different patterns on hepatic enzyme activities and serum biochemical ...
Tubulin>GAL80tswas present in all flies, to limit the time window in which Kir2.1* or Kir2.1*Mut transgenes were expressed (Methods). The 18 °C control was not shifted to 31 °C before the assay and thus expression of Kir2.1* or Kir2.1*Mut was not induced. All egg-laying exp...
Hisat2 and StringTie were used to align clean reads to the reference genome (GRCH38) and profile gene expression, respectively. HTSeq-count was used to count the reads aligned to each gene then differentially expressed genes (DEGs) (S sensitive vs resistant) were defined by edgeR (fold ...
[85]. At high RH, droplet size follows very well the approximate expression given by (8). At around RH = 40%, however, the radius suddenly drops, indicating an efflorescence transition. Consequently, the difference in the sizes of droplet residues at 30% and 50% RH is almost 40%—...
Normalization of gene expression data refers to the comparison of expression values using reference standards that are consistent across all conditions of an experiment. In PCR studies, genes designated as "housekeeping genes" have been used as internal reference genes under the assumption that their ...