From 30 min to 3 h after occlusion, 0.5 ml of the double-labeled blood was injected in 5 s from the femoral vein. Thirty seconds after the injection, the brain was removed and fixed in 95% ethanol. Coronal sections, 25 渭m thick, were mounted in glycerol and examined under the ...
The behaviors of MZ B cells were observed with intravital two-photon microscopy inCd19−/−mice reconstituted with Ub-GFP+B cells. CTV-labeled naive B cells were used to identify follicles. PKH26-labeled RBCs were intravenously injected 3 h before imaging, and 70-kD Texas Red dextran w...
Hemolysis is a cardinal feature of hemolytic uremic syndrome (HUS) and during hemolysis excess arginase 1 is released from red blood cells. Increased arginase activity leads to reduced L-arginine, as it is converted to urea and L-ornithine, and thereby r
Red blood cells (RBCs) passing through heart pumps, prosthetic heart valves and other cardiovascular devices undergo early senescence attributed to non-physiologic forces. We hypothesized that mechanical trauma accelerates aging by deformation of membrane proteins to cause binding of naturally occurring IgG...
while leukocytes were not magnetically labeled. Therefore, fNRBCs could be well separated. The results showed that each milliliter of pregnant women's peripheral blood samples contained an average of 37.44 target cells. Compared with the results of previous studies, the number of cells enriched by...
Considering the surface charge of outer leaflet of eukaryotic/human red blood cell membrane and bacterial membrane, experiments were performed with zwitterionic, PC/Chol and negatively charged, PC/PG lipid vesicles respectively. Experiments with NBD-labeled analogs clearly suggested that although H-88 an...
“No, really. Not tamper-proof,obviously, but tamper-resistant— you’d need a tunneling microscope or a vat of Freon to extract the keys from the chip. And every chip has its own keys, so you’d need to do this for every chip, which doesn’t, you know,scale.So there’s this ch...
Under a Creative Commons license open accessOur recent studies identified juvenile hormone (JH) and nutrition as the two key signals that regulate vitellogenin (Vg) gene expression in the red flour beetle, Tribolium castaneum. Juvenile hormone regulation of Vg synthesis has been known for a long ...
RBCs were induced under high temperature (48 °C) to prepare stressed RBCs. Fluorescence-activated cell sorting (FACS) was used to analyse reactive oxygen species (ROS) generation, intracellular Ca2+ concentration and membrane phosphatidylserine (PS) externalization of RBCs. 18F-FDG was used to ...
Whole blood flow cytometry analysis of HbAA (normal hemoglobin) and HbSS (homozygous sickle cell hemoglobin) RBCs using fluorescently labeled plant lectins, detailed in “Methods” section. Vertical axes show normalized geometric mean fluorescence (gMFI). Symbols of terminal carbohydrates detected by pla...