Average cell diameter was determined with a phase-contrast microscope (Leitz Wetzlar dialux 20EB, E. Leitz GmbH Wetzlar, F.R.G.) with calibrated eye piece ocular (magnification 1,000×) based on the measurement of 100 cells. RESULTS The dependence of blood platelet adherence on the ...
Under a Creative Commons license open accessHighlights • Standardization for the evaluation of CMI responses in HIV-1 patients is needed. • A higher quality of cells (viability/recovery) was seen for shorter processing times. • Overnight in vitro stimulation improves the HIV-1 specific CD8...
(A,B) Time-lapse snapshots taken in the same location at t = 2 minutes (A) and t = 7 minutes (B) after extraction under an inverted transmitted-light phase-enhanced microscope. Aggregation of cells connected with an amorphous dark grey material is visible (red arrow). As ...
We recently developed a method to study the microvascular perfusion within the brain parenchyma using fluorescence-labeled plasma [1]. The method was extended in the present study to visualize circulated plasma as well as red blood cells (RBC) simultaneously in the same brain slice [2]. After ...
Furthermore, this smart optofluidic system can be extended to disease diagnosis accompanied by abnormal morphology and mechanics of blood cells, etc.; it can also be used to evaluate the recovery of patients with blood diseases after treatment. Moreover, this system can be expanded into in vitro...
Red blood cells (RBCs) were counted using the method described by [28] with a Bright-Line Hemocytometer (Neubauer enhanced, Germany). Hemoglobin (Hb) levels were measured calorimetrically, as outlined by [29]. Hematocrit (Hct) was calculated following the method of [30]. The levels of MCV...
a dark purple color when viewed under a microscope. the lymphocytes are mainly responsible for the body's immune response against viral infections. lymphocytes can be classified as t cells, b cells, or natural killer cells. t cells come from the bone marrow but mature and develop in the ...
for 2h and stained with dapi. the samples were observed under a fluorescence microscope. proliferation, apoptosis, and migration analysis of pbmcs cultivated with reganxb18 or r eganxb20 pbmcs (1 × 10 6 cells/100μl/well) were seeded into 96-well plates and incubated with r eg an...
the situation changes under inflammatory conditions. Thus, molecular cues associated with inflammation (pathogens, autoimmunity, cancer) lead to a significant increase in the permeability of blood vessels, such that immune effector proteins (e.g. antibodies) and cells can enter the affected tissue [29...
After each incubation period of either 30 min or 1 h, the parasites were removed washed, gently centrifuged, transferred into fresh media, and visually inspected under a microscope to ensure viability and intact morphology. At each time point all media were collected, including the wash media. ...