Real-time PCR analyses of genera Veillonella and Streptococcus in healthy supragingival plaque biofilm microflora of childrenchildrenplaque biofilmreal-time polymerase chain reactionStreptococcusVeillonellaSince Veillonella species obtain the energy for their growth by fermenting organic acids, e.g., lactate, ...
Similar to other real-time PCR software, CFX Maestro software offers several analysis modules, including quantification, melt curve, gene expression, allelic discrimination, and end-point analyses. An example of how to analyze a gene expression experiment in CFX Maestro software is shown below. ...
Two different methods of presenting quantitative gene expression exist: absolute and relative quantification. Absolute quantification calculates the copy number of the gene usually by relating the PCR signal to a standard curve. Relative gene expression presents the data of the gene of interest relative ...
To assess chondrogenesis, gene expression analyses using RT-PCR were conducted on chondrogenic-MSCs using cartilage-specific phenotypic markers at pre-determined time points. RESULTS: Flow cytometry analyses confirm the presence of MSCs and absence of haematopetic stem cells. The gene expression level ...
In this report, we presented four models of statistical analysis of real-time PCR data and one procedure for data quality control. SAS programs were developed for all the applications and a sample set of data was analyzed. The analyses with different models and programs yielded the same estimati...
Additionally, BLAST analyses were performed to verify their specificity (Fig. 1). The PCR products of the female adults were sequenced by an ABI 3730 automated sequencer (Applied Biosystems, Foster City, CA, USA) in both directions. The obtained sequences were submitted to GenBank database (...
摘要: Design of primers and probes is one of the most crucial factors affecting the success and quality of quantitative real-time PCR (qPCR) analyses, since an accurate and reliable quantification depends...关键词: Quantitative real-time PCR Primers Probes Software and databases Validation Reverse...
examined in eight tissues of Atlantic salmon (Salmo salar), to determine the most suitable genes to be used in quantitative real-time RT-PCR analyses. ... PA Olsvik,KK Lie,AE Jordal,... - 《Bmc Molecular Biology》 被引量: 987发表: 2005年 Quantitative real-time RT-PCR - a perspective...
The amount of PCR products (amplicons) can be detected in real-time in both methods by measuring fluorescence levels from the cycle threshold (Ct). Both types of analyses are potentially fast and sensitive but the SYBR Green I dye is more cost-effective than the TaqMan probe. Recently, SYBR...
In order to perform these analyses, a real-time PCR assay was developed. The stability of the φLC3 prophage was found to vary with the growth phase of its host L. lactis IMN-C1814, in which the induction rate increased during the exponential growth phase and reached a maximum level when...