Real-time PCR analyses of genera Veillonella and Streptococcus in healthy supragingival plaque biofilm microflora of childrenchildrenplaque biofilmreal-time polymerase chain reactionStreptococcusVeillonellaSince Veillonella species obtain the energy for their growth by fermenting organic acids, e.g., lactate, ...
Similar to other real-time PCR software, CFX Maestro software offers several analysis modules, including quantification, melt curve, gene expression, allelic discrimination, and end-point analyses. An example of how to analyze a gene expression experiment in CFX Maestro software is shown below. ...
Two different methods of presenting quantitative gene expression exist: absolute and relative quantification. Absolute quantification calculates the copy number of the gene usually by relating the PCR signal to a standard curve. Relative gene expression presents the data of the gene of interest relative ...
Long range PCR and breakpoint sequencing analyses validates the triplex assay In order to further validate the triplex assay, we selected a representative group of single cell preparations (a cell with no detectable deletion,MT-ND1deletion and combinedMT-ND1 + MT-ND4deletion) and subjected the...
摘要: Design of primers and probes is one of the most crucial factors affecting the success and quality of quantitative real-time PCR (qPCR) analyses, since an accurate and reliable quantification depends...关键词: Quantitative real-time PCR Primers Probes Software and databases Validation Reverse...
Samples from 595 blood donors were collected in seven haemotherapy centres in northern Brazil located in areas at risk for malaria transmission, and the analyses were performed by real-time PCR with TaqMan probes on 7500 Real-Time PCR Systems, to genotype the mitochondrial DNA region specific to...
The same analyses were performed for the hMPV detection assay. As shown in Fig. 5, for two different InfB and hMPV targets using two different URs (different mediator hybridization sequences), the optimal MP design according to the target value magnitude demonstrated reduced detection limits ...
The amount of PCR products (amplicons) can be detected in real-time in both methods by measuring fluorescence levels from the cycle threshold (Ct). Both types of analyses are potentially fast and sensitive but the SYBR Green I dye is more cost-effective than the TaqMan probe. Recently, SYBR...
Stage I gametocytes are morphologically indistinguishable from pigmented asexual trophozoites, and thus cannot be identified in peripheral blood by microscopic examination. Transcriptome analyses have led to the identification of transcripts upregulated before morphological differentiation into stage II gametocytes...
Evaluation of potential reference genes in real-time RT-PCR studies of Atlantic salmon examined in eight tissues of Atlantic salmon (Salmo salar), to determine the most suitable genes to be used in quantitative real-time RT-PCR analyses. ... PA Olsvik,KK Lie,AE Jordal,... - 《Bmc Molecu...