QIAGEN 替您完成 real-time PCR 引 物设计和反应优化 随着荧光定量 PCR 仪的普及,荧光定量 PCR 也开始飞入寻常实验室了.不过 real-time PCR 可不 像终端 PCR 那么轻巧.常规 PCR 只要扩出条带就算行,条带深点浅点偶尔有点副产物问题不太大,而 实时定量 PCR 既然目标是定量,自然就要严谨得多.稍有偏差都会...
This chapter shows the mathematics involved in quantitating the amount of PCR product made during the real-time PCR process using intercalating fluorescent dyes and the TaqMan assay. The methods discussed include absolute quantification using a standard curve, relative quantification using the comparative ...
实时荧光定量PCR(Real—time PCR)方法检测大豆根腐病菌(摘要) 茄腐镰刀菌(Fusarium solani f.sp.Glycines,FSG)是一种土传真菌病害,主要侵染大豆根部并且引起大豆猝死综合征(sudden death syndrome,SDS),这是一种广泛的,具有破坏性... Li,Hartman,Domier,... - 《大豆科技》 被引量: 0发表: 2010年 实时...
Real-Time PCR webinars Genetic Analysis for All webinars TaqMan Gene Expression Assays brochure TaqMan Assays infographic Using TaqMan Endogenous Control Assays to Select an Endogenous Control for Experimental Studies The design process of quantitative TaqMan gene expression analysis tools R...
Real-Time PCR Troubleshooting Tool Protocol: TaqMan Gene Expression Assays Quick Reference Card: TaqMan Gene Expression Assays Application Note: Using TaqMan Endogenous Control Assays to Select an Endogenous Control for Experimental Studies The Design Process for a New Generation of Quantitative Gene Expres...
The sensitivity of the real-time PCR procedure was evaluated at 500 HCMV DNA copies per ml of sera. The use of an internal control concomitantly processed during the HCMV quantification did not alter the sensitivity of the procedure, and was relevant for the detection of putative PCR ...
Background & methodology Real-time quantitative PCR is the reliable detection and measurement of products generated during each cycle of the PCR process, which are directly proportional to the amount of template prior to the start of the PCR process. Holland and coworkers demonstrated that the ...
笔记 Applied Biosystems StepOne™ Real-Time PCR System 基因分型实验入门指南 13 第 2 章设计实验 定义实验属性 定义实验属性 在实验属性屏幕上,输入实验标识信息,然后选择要设计的实验类型。 完成此屏幕 1. 单击 Experiment Name (实验名称)字段,然后输入Genotyping Example (基因分型示例)。 2. 单击 Barcode...
From Ct analysis of your real-time PCR gene expression experiments, to allele cluster analysis of TaqMan® SNP Assay data, to CNV copy number determination, to HRM-curve generation, we create software that enables you to easily analyze data from
From Ct analysis of your real-time PCR gene expression experiments, to allele cluster analysis of TaqMan® SNP Assay data, to CNV copy number determination, to HRM-curve generation, we create software that enables you to easily analyze data from