The number of 3β-HSD positive cells are expressed relative to the percentage of the tile that was occupied by seminiferous cords. Normally distributed data was analysed using one-way ANOVA or Student’s t-test, whilst non-parametric data was analysed using the Mann–Whitney test. All ...
FiO2, fraction of inspired oxygen; MAP, mean airway pressure; PEEP, positive end- expiratory pressure; PIP, peak inspiratory pressure; Pplat: plateau pressure = pressure measured at the end of inspiration and before start of expiration. Table 2. Arterial blood gas parameters after 1 or 6...
In a previous study of our group, AP-1 components c-Jun and c-Fos showed good positive correlation with inflammatory cytokines levels in rat brain[80]. Therefore, we analyzed the relative content of c-Jun and c-Fos using western blot method. However, only results acquired from striatum ...
To identify which cells express MANF protein after stroke, we used double immunofluorescence staining on sections from the post-stroke rat brain. MANF and CD68 positive signals were found to be co-localized in the same cells in the post-stroke rat brain verified by confocal microscopy. In the...
ATP content in rat retina, before treatment and after 7, 20 and 45 days of streptozotocin administration.Ixchel OsorioPazSalvador UribeCarvajalRocío Salceda
Immunofluorescence results showed double-positive fibrocytes from day 3 to day 12 (Figure 1B). Furthermore, the number of fibrocytes was increased, and purified fibrocyte population represented up to 75.6% as assessed by flow cytometry after 12 days (Figure 1C and 1D). Purified fibrocytes still...
Sample introduction and ionization was electrospray ionization in the positive ion mode. The cone voltage was 20 V and the source temperature was 140 °C. Ions were collisionally activated at a collision energy of 35 eV and at an indicated argon pressure of 2.0 × 10−3 torr. To assay ...
The optic nerves were not positive for the expression of iNOS. In conclusion, this study provides no evidence that lamotrigine is neuroprotective for RGC axons after four weeks of experimental ocular hypertension in the rat, in a model where axonal degeneration occurs in the absence of iNOS ...
Normal rat lungs were used as positive control since RAGE is highly expressed in the lungs59. The primary antibody was diluted (1:80) with 1% BSA-PBS and normal rabbit serum (diluted 1:60) pre-treated with excessive soluble RAGE was used as negative control. Other processes were similar ...
and mucus secretion area ratio was determined according to a previous study52. Results were expressed as the ratio of mucus stain positive area/villus area. The density of goblet cells per villus, expressed as number of goblet cell/mm villus was also measured29. Five measures per animal were ...