2. BioTNT PCR array的适用在哪里? 回答:适用现有的通路研究; 适用少样本或中等样本,多基因的QPCR 检测; 在低样本或中等样本的情况下,费用和时间远小于自行建立QPCR实验; 使用方便,操作简单,结果稳定性好; 3. BioTNT PCR array的优点是什么? 回答:灵敏度高,样本使用量低; 线性范围广,可同时检测表达水平差异...
After osteoarthritis induction by MIA, the levels of MMP1 and MMP13 significantly increased to 3.6- and 3.9-fold, respectively, in contrast to the control group (Fig. 5). Subjecting the rats to swimming and curcumin individual treatments for 4 weeks attenuated the increase resulting from MIA ...
Cell apoptosis was quantified with Hoechst 33258 staining and TUNEL assay. The level of 14,15-DHET, a stable metabolite of 14,15-EET, was assessed with ELISA. Results: Angiotensin II (10−6 mol/L) significantly decreased the expression of calcium-regulating molecules (SERCA2a, PLB, RyR2 ...
H&E staining for neuronal damage, TUNEL assay for detection of apoptotic cells, immunofluorescence, ELISA protocol for inflammatory cytokines and 8-OHdG determination and western blot analysis to determine the effect of HRS in LPS-induced septic rats. Rats treated with HRS showed decreased TNF-α ...
International journal of cardiology A sirtuin 1/MMP2 prognostic index for myocardial infarction in patients with advanced coronary artery disease pubmed:28043667 International Journal of Cardiology Serum concentrations and gene expression of sirtuin 1 in healthy and slightly overweight subjects after caloric...
Similarly, expression levels of MMP-2 were also found to be significantly reduced in plant-treated groups (methanol extract: 1.391 ± 0.292; n-hexane fraction: 1.307 ± 0.16; ethyl acetate fraction: 1.265 ± 0.105) compared to those in the positive control group. Whereas, similar significant ...
decreased in aged skin and increased by RA. The levels of NF-κB/AP-1 and MMP1/2/3/9 were increased in aged skin and decreased by RA. The levels of TGF-β, CTGF, and collagen I/III were decreased in aged skin and increased by RA. The expression of laminin and nidogen and ...
The activities as well as expression of MMP-2 and MMP-9 were measured by MMP activity ELISA kits and Western blotting, respectively. Their possible regular pathways, e.g., p38 MAKP, ERK1/2, Akt, and NF-κB, were all analyzed after the treatment of DEHP. 2. Results and Discussion 2.1...