产品名称: RAS ACTIVATION ASSAY KIT .0 订购此产品 供应商: Millipore 规格: EA 目录价: 7944 库存状态: 三周到货 CAS编号: 应用范围: 生化实验 种属来源: 相关信息: Ras Activation Assay Kit Recommended Replacement for: SGT435 Close PRODUCT FAMILY INFORMATION RAS Antibodies, Kits, Proteins & Pla...
I. TEST PRINCIPLE 2 II. Ras BACKGROUND 3 III. SPECIES REACTIVITY 4 IV. STORAGE AND STABILITY 4 V. KIT COMPONENTS, PRECAUTIONS and TECHNICAL NOTES 4 VI. PREPARATION OF BUFFER/SOLUTIONS 5 VII. CELL LYSATE SAMPLE PREPARATIONI TESTIV STORAGEV KITVII CELLDirect.millipore.com...
CAS号: 英文名: Ras Activation Assay Kit 英文别名: Ras Activation Assay Kit 中文名: 中文别名: CBNumber: CB07244780 分子式: 分子量: 0 MOL File: Mol file 化学性质 安全信息 用途 供应商 4 Ras Activation Assay Kit化学性质
Ras activation assay Ras activities were analyzed using the Ras Activation Assay Kit (Millipore, Billerica, MA, USA), according to the manufacturer’s instructions with some modifications. Active Ras was pulled down with Raf-1 Ras binding domain (RBD)-conjugated agarose beads (Millipore) at 4 °...
Ras activity was determined using a Ras activation assay kit (Millipore, Watford, Hertfordshire, UK). Briefly, cells were harvested 48 h after transfection in an Mg-containing lysis buffer (MLB). 500 μg of cell lysate from each sample was incubated with 10 μg of Raf-1 RBD agarose...
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Ras GTP levels were determined by a Ras activation assay kit (Upstate, Millipore) according to the manufacturer's directions, or by previously published protocols [24, 51]. Lysates (1 mg of total cell protein in each sample) were incubated with 10 μg Raf-1-RBD for 45 min at 4°C ...
小鼠成神经细胞瘤RAS病毒(v-ras)癌基因同源物elisa试剂盒英文名称:Mouse GTPase NRas(NRAS)ELISA kit产品简称:v-raselisa试剂盒英文简称:v-ras elisa Kit采用的全是原材料研,发灵敏性高,高效性,原装抗体,吸附均匀、吸附性好,回收利用率高、可靠性强,无效包退包换,公司提供免费代测服务,各种种属ELISA试剂盒产品齐...
大鼠Ras相关C3肉毒菌毒素底物1(Rac1)检测试剂盒酶联免疫吸附试验(ELISA)因其操作简单,灵敏度高,特异性好,经济安全等特点而在临床上广泛应用,但是由于其操作步骤复杂,一般包括试剂准备,样本收集,加样,温育,洗涤,显色,比色,结果判定等,其中任何一项操作不当都会对检测结果产生很大影响。因此,必须加强ELISA检测的全面...
However, during the conducted studies, it was discovered that the G12V mutation does not always result in AKT and ERK activation. A SW480AKTlack cell subline was obtained, which, despite the presence of this mutation, did not show increased AKT and ERK pathway activity. Nevertheless, the cell...