The advantage of this type of labeling is that all regions of the template are found in the fragment mix. This method is less problematic than nick translation, because the primer concentration, unlike the DNase concentration, does not represent a critical element. Random priming is used for rad...
The crosslinked proteinosomes were phase transferred into water, and the addition of biotin-functionalized or dsDNA strands led to DNA template accumulation and stable retention up to 200 base pairs (bp) (ref. 37). In previous work37, we have characterized the proteinosome membrane and ...
We compared two advanced random test generators, a Feedback-Directed Random Test Generator (FDR) and a Feedback-Controlled Random Test Generator (FCR), for an automated software testing tool in Python 2.x, the Template Scripting Testing Language (TSTL).An FDR generates test inputs incrementally...
template-switching strategy for the second strand cDNA synthesis. The template-switching strategy could be affected by the complex structure of RNAs and fail to capture interrupted cDNAs42. Thus, we adapted a dA tailing strategy for efficient second-strand cDNA synthesis, as non-template dA can ...
template-switching strategy for the second strand cDNA synthesis. The template-switching strategy could be affected by the complex structure of RNAs and fail to capture interrupted cDNAs42. Thus, we adapted a dA tailing strategy for efficient second-strand cDNA synthesis, as non-template dA can ...
Ray et al. evaluated the regional alterations in the ADC of cortical gray and white matter and subcortical structures, which are known to be involved in MCI, stating that ADC from gray and white matter of different brain regions could be analyzed by applying an automated template-masking method...
All the MR brain images were aligned along the line that passes through the anterior and posterior commis- sures of the brain, corrected for their bias field, and finally spatially normalized to the MNI152 template space using affine transformation35. Scientific Reports | (2019) 9:16839 | ...
Briefly, Poly(A)+ mRNA from extracted total RNA were converted to cDNA decorated with universal adapters, sample-specific barcodes and unique molecular identifiers (UMIs) using a template-switching reverse transcriptase. Decorated cDNA from multiple samples were then pooled, amplified (10 PCR cycles...
Thetet(M) PCR mixture (50 µL final volume) contained 1X PCR buffer (Promega), 3 mM MgCl2, 0.2 mM dNTPs, 1 μM of each primers (IntMtet1 and IntMtet2), 1 U of HotStart G2 DNA polymerase (Promega), and 5 μL of DNA template. Amplification was performed as descr...
The invention may be used in many areas such as drug discovery, functionalized substrates, biology, proteomics, combinatorial chemistry, and any assays or multiplexed experiments. Examples of common assays are SNP (single nucleotide polymorphism) detection, DNA/genomic sequence analysis, genotyping, gene...