In this study, we developed a droplet-based, high-throughput, and high-sensitivity single-microbe RNA-seq method, named smRandom-seq, with the pipeline of bacteria preparation, reactions in situ, droplet barcoding, and library preparation. We provided sufficient and systematic evidence to prove the...
Briefly, Poly(A)+ mRNA from extracted total RNA were converted to cDNA decorated with universal adapters, sample-specific barcodes and unique molecular identifiers (UMIs) using a template-switching reverse transcriptase. Decorated cDNA from multiple samples were then pooled, amplified (10 PCR cycles...
Generalizing these ideas and performing them efficiently leads to the max-product algorithm, a general template for performing such computations exactly in arbitrary tree-structured graphs. The max-product or max-sum algorithm The max-product algorithm can be used to find the most probable explanation...
The present invention relates to an automated analytical apparatus, and more particularly, concerns an automatic analyzer for the chemical or immunochemical testing of substances on a random access basis. 2. Description of the Prior Art. There are many known and available analyzers for the chemical,...
Some template-based methods go beyond simply searching for contacts in existing template structures and incorporate machine learning techniques into their predictions. For example, Wu and Zhang were able to combine SVMs with data from multiple threading methods into a contact predictor which they named...
Thetet(M) PCR mixture (50 µL final volume) contained 1X PCR buffer (Promega), 3 mM MgCl2, 0.2 mM dNTPs, 1 μM of each primers (IntMtet1 and IntMtet2), 1 U of HotStart G2 DNA polymerase (Promega), and 5 μL of DNA template. Amplification was performed as descr...
All the MR brain images were aligned along the line that passes through the anterior and posterior commissures of the brain, corrected for their bias field, and finally spatially normalized to the MNI152 template space using affine transformation35. ...
While this concept is interesting for platform development, as the "template" vector has to be produced only once, several process optimizations are still required. For example, the low incorporation of the VP2-tag into the AAV particles has to be addressed, as this is a critical determinant ...
The final reaction volume was 12.5 µL, including 1 µL template cDNA and a primer concentration of 500 nM. PCR conditions were as follows: 20 s at 95 ◦C, 40 cycles of 3 s at 95 ◦C, 1 min at 60 ◦C, followed by a melting curve analysis, with a temperature gradient ...