In this study, we developed a droplet-based, high-throughput, and high-sensitivity single-microbe RNA-seq method, named smRandom-seq, with the pipeline of bacteria preparation, reactions in situ, droplet barcoding, and library preparation. We provided sufficient and systematic evidence to prove the...
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Screening ofM.hoministransformants by PCR Thetet(M) PCR mixture (50 µL final volume) contained 1X PCR buffer (Promega), 3 mM MgCl2, 0.2 mM dNTPs, 1 μM of each primers (IntMtet1 and IntMtet2), 1 U of HotStart G2 DNA polymerase (Promega), and 5 μL of DNA te...
All the MR brain images were aligned along the line that passes through the anterior and posterior commissures of the brain, corrected for their bias field, and finally spatially normalized to the MNI152 template space using affine transformation35. Atlas selection and registration To reduce the co...